中文摘要：

瞄准：为肠炎沙门氏菌识别并且理解常规分发模式(S。enteritidis ) 在在在 3 wk 时期上的口头的挑战以后的老鼠的内脏。方法：试金 S 基于 serovar 特定的 DNA 定序。从 GenBank 的 enteritidis，和 serovar 特定的即时、基于荧光的量的聚合酶链反应(FQ-PCR ) 为 S 的察觉被开发。enteritidis。我们使用了这试金检测 S 的 genomic DNA。包括心，肝，怒气，肾，胰，和胆囊，在血和内脏的 enteritidis 分别地从在在不同时间的口头的挑战以后的老鼠指。结果：结果证明怒气在 12 h 柱子接种(PI ) 是积极的，并且血在 14 h PI。有机体在 16 h PI 在肝和心被检测，胰在 20 h PI，和最后的器官是积极的证明阳性结果在 22 h PI 是肾和胆囊。S 的拷贝数字。在每纸巾的 enteritidis DNA 在 24-36 h PI 到达了一座山峰，与包含 S 的高集中的肝和怒气。enteritidis 而血，心，肾，胰，和胆囊有低集中。S。enteritidis 人口开始减少并且不在 3 d PI 是可检测的，但是没有引起明显的症状，仍然为脾是直到在胆囊，为肝的 2 wk，和 3 wk 的 12 d PI 的现在。结论：结果为理解 S 的生活史提供了重要数据。在内脏的 enteritidis，并且证明肝和怒气可以是为在口头的挑战以后在很长时间作为 commensa 建立自己的主要地点。有趣地，它可以是报导的第一次胆囊是为 S 的马车的一个地点。在 12 d 时期上的 enteritidis。这研究将帮助理解 S 的行动的机制。enteritidis 感染在活体内。

英文摘要：

AIM： To identify and understand the regular distribution pattern for Salmonella enteritidis （S. enteritidis） in the internal organs of mice after an oral challenge over a 3 wk period. METHODS： Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction （FQ-PCR） were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively. RESULTS： The results showed that the spleen was positive at 12 h post inoculation （PI）, and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis, whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations. S. enteritidis populations began to decrease and were not detectable at 3 d PI, but were still present up to 12 d PI in the gallbladder, 2 wk for the liver, and 3 wk for the spleen without causing apparent symptoms. CONCLUSION： The results provided significant data for understanding the life cycle of S. enteritidis in the internal organs, and showed that the liver and spleen may be the primary sites for setting itself up as a commensa over a long time after oral challenge. Interestingly, it may be the first time reported that the gallbladder is a site of carriage for S. enteritidis over a 12 d period. This study will help to understand the mechanisms of action of S. enteriCdis infection in vivo.