中文摘要：

瞄准：检测肠炎沙门氏菌(S。enteritidis ) 在在感染的鸭纸巾的石蜡片和抗原地点。方法：兔子与净化的杆菌被使免疫获得 S。enteritidis 特定的抗体，然后被辛酸铵的硫酸盐方法提取，通过 High-Q 净化了列。一个间接免疫荧光灯的染色方法(IFA ) 被建立检测 S。在石蜡片的 enteritidis 抗原。检测 S。在试验性地感染 S 的小鸭的每机关纸巾的 enteritidis。enteritidis。结果：Garder 的腺，心，肾，脾，肝，大脑，回肠，空肠，从 S 的赘言作家的囊。enteritidis 试验性地感染了小鸭积极或强烈积极，并且 S。主要在感染的房间细胞质散布的 enteritidis 抗原。结论：IFA 是在检测 S 的一个直觉主义、敏感、特定的方法。在石蜡的 enteritidis 抗原切，并且它是在诊断和 S 的抗原地点的一个好方法。enteritidis。我们也断定 Garder 的腺，心，肾，怒气，肝，回肠，空肠是在 S 的靶器。鸭的 enteritidis 感染，和 S。enteritidis 是一个细胞内部的寄生细菌。

英文摘要：

AIM： To detect Salmonella enteritidis （S. enteritidis） in paraffin slices and antigen location in infected duck tissues. METHODS： The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method （IFA） was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis. RESULTS： The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm. CONCLUSION： IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.