中文摘要：

目的探讨蝙蝠葛碱（DAU）对阿尔茨海默病（AD）小鼠海马晚期糖基化终产物受体（RAGE）和核转录因子（NF）-κBp65的影响。方法雄性小鼠随机分成对照组、模型组、多奈哌齐组（0.001 g/kg）及DAU高、中、低剂量组（2.4、1.2、0.6 g/kg）。小鼠双侧海马微量注射β-淀粉样蛋白（Aβ）（1~42）（2 g/L）和腹腔注射D-半乳糖（180 mg/kg）诱导AD小鼠,连续治疗35 d后取材。采用Morris水迷宫（MWM）检测AD小鼠学习记忆能力,酶联免疫吸附（ELISA）检测海马白细胞介素（IL）-1β、IL-6含量,免疫组化（IHC）、实时定量（RT）-PCR和Western印迹检测海马RAGE和NF-κBp65表达。结果与模型组比较,DAU高、中剂量明显改善AD小鼠学习记忆能力,明显降低海马IL-1β、IL-6、RAGE和NF-κBp65水平（均P〈0.05）。结论 DAU通过抑制海马RAGE和NF-κBp65表达来改善AD小鼠学习记忆能力,以此在AD治疗中发挥重要作用。

英文摘要：

Objective To explore the effects of dauricine（ DAU） on receptor for advanced glycation end products（ RAGE） and nuclear transcription factor（ NF）-κBp65 of the hippocampus in Alzheimer＇s disease（ AD） mice. Methods Male mice were randomly divided into control,model,donepezil（ 0.001 g/kg）,DAU high-dose（ 2.4 g/kg）,DAU medial-dose（ 1.2 g/kg）,DAU low-dose（ 0.6 g/kg） groups.The AD model mice were induced by microinjection of incubated amyloid beda91 ~ 42）（ 2 g/L） into the dorsal blade of and intraperitoneal injection of D-galactose（ 180 mg/kg） in the dentale gyrus in the bilateral hippocampus. Morris water maze was used to observe the learning and memory ability of AD mice. Enzyme-linked immunosorbent assay（ ELISA） was used to determine the contents of IL-1β and IL-6. By immunohistochemistry（ IHC）,real time（ RT）-PCR and Western bolt were used to test the contents of RAGE and NF-κBp65 of the hippocampus in AD mice.Results Compared with those of model group,DAU high-dose and medial-dose significantly improved the learning and memory ability of AD mice（ P〈0.05）. DAU high-dose and medial-dose decreased the levels of IL-1β,IL-6,RAGE and NF-κBp65 in the hippocampus（ P〈0.05）.Conclusions DAU could improve the ability of learning and memory of AD mice. DAU plays a certain role in the treatment of AD through inhibiting RAGE and NF-κBp65.