一种新奇敏感半量的病毒察觉技术作为一个例子用呼吸 syncytial 病毒(RSV ) 被开发,通过散布入侵病毒的宿主细胞的表面状态的成像的黑暗地的光。在这个方法, anti-RSV-antibody 修改了金 nanoparticles (AuNPs ) 能通过特定的抗体抗原绑定在感染的主人房间的房间膜上与入侵的病毒绑。然后,宿主细胞能被在黑暗地的轻散布显微镜学下面散布 AuNPs 的性质的局部性的表面电浆子回声光想象,它能进一步习惯于半确定入侵的病毒。
A novel sensitive semi-quantitative virus detection technique was developed using the respiratory syncytial virus (RSV) as an example, through dark-field light scattering imaging of the surface state of the virus- invaded host cells. In this method, anti-RSV-antibody modified gold nanoparticles (AuNPs) could bind with the invading virus on the cell membrane of the infected host cells through the specific antibody-antigen binding. Then, the host cells could be imaged by the localized surface plasmon resonance light scattering properties of AuNPs under a dark-field light scattering microscopy, which could be further used to semi-quantify the invading virus.