中文摘要：

Combining the unique optical properties ofmetal nanoparticles and the specific recognition of apt-amer, aptamer-nanoparticle conjugates have been exten-sively used in a wide range of applications, particularlymultifunctional nanoparticles for cell detection andmolecular imaging. Conventional conjugates prepared bychemisorption of monothiol-modified oligonucleotidesonto nanoparticle surfaces suffer from a lack of stabilitywhen exposed to a variety of small molecules. If silver isused in place of gold, then this lack of stability is evenmore pronounced. In this study, we reported here theeffective and facile strategy of preparing stable silvernanoparticle-aptamer conjugates by in situ generation ofstrong metal affinity capping ligands, dithiocarbamatesmodified anti-priori protein aptamer. The conjugates pro-duced are stable and can withstand NaC1 concentration at0.25 mol/L. Meanwhile, they could be applied in the cel-lular prion protein imaging successfully.

英文摘要：

Combining the unique optical properties ofmetal nanoparticles and the specific recognition of apt-amer, aptamer-nanoparticle conjugates have been exten-sively used in a wide range of applications, particularlymultifunctional nanoparticles for cell detection andmolecular imaging. Conventional conjugates prepared bychemisorption of monothiol-modified oligonucleotidesonto nanoparticle surfaces suffer from a lack of stabilitywhen exposed to a variety of small molecules. If silver isused in place of gold, then this lack of stability is evenmore pronounced. In this study, we reported here theeffective and facile strategy of preparing stable silvernanoparticle-aptamer conjugates by in situ generation ofstrong metal affinity capping ligands, dithiocarbamatesmodified anti-priori protein aptamer. The conjugates pro-duced are stable and can withstand NaC1 concentration at0.25 mol/L. Meanwhile, they could be applied in the cel-lular prion protein imaging successfully.