中文摘要：

一种新类型高度选择 标记aptamer 荧光灯硅石 nanoparticles [ Apt-tris ( 2,2-bipyridyl )钌( II )@ SiO2 NP ]被使用 tris ( 2,2-bipyridyl )的延长荧光一生钌建筑群通过反向的微乳液方法准备钌( II )( Ru ( bpy ) 3 2+)作为为细胞的 prion 蛋白质成像荧光采购原料。调查证明上级为 bioconjugation 强壮的荧光，低毒性，和容易的表面修正有利的 3 2+@ SiO2 NP 拥有了的最新准备的 Ru (bpy ) 。房间成像实验显示了 3 2+@ SiO2 NP 有的那 Apt-Ru ( bpy )大趋势到人的骨头髓 neuroblastoma 房间( SK-N-SH 房间)，后来，他们能在房间的表面上表示大量 prion 蛋白质，当在 HeLa 房间这现象为 HeLa 房间不能表示 prion 蛋白质的原因消失了时。

英文摘要：

A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris（2,2＇-bipyri- dyl）ruthenium（II）@SiO2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris（2,2＇-bipyri- dyl）ruthenium（II） （Ru（bpy）2＋） as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru（bpy）32＋@Si02 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt- Ru（bpy）32＋@SiO2 NPs had great tendency to human bone marrow neuroblastoma cells （SK-N-SH cells）, since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.