中文摘要：

双分子的荧光互补(BiFC ) 广泛地在最近的年里在 proteinprotein 相互作用(PPI ) 的分析被使用了。有象便利和在房间的 PPI 的直接可视化那样的 BiFC 的许多著名优点。然而， BiFC 有一普通限制：分开的非荧光灯的碎片能是自发地自我装配的进未经触动的蛋白质，它导致假积极的结果。在这研究，一双互补碎片(sfGFPN 和 sfGFPC ) 被切开在 214 和 215 氨基酸残余之间的 superfolder GFP (sfGFP ) 构造，并且 sfGFPC 被指导地点的基因 mutagenesis 变异减少否定控制的信号。我们的结果证明在 sfGFPC (sfGFPC (m12 )) 的变化能有效地减少否定控制的信号。因此，我们为 PPI 的分析提供一个改进 BiFC 工具。，推进自己组装问题是为 BiFC 的申请的一个普通缺点，我们的研究为另外的 BiFC 候选人蛋白质向可行策略提供一样的问题。

英文摘要：

Bimolecular fluorescence complementation （BiFC） has been widely used in the analysis of protein-protein interactions （PPIs） in recent years. There are many notable advantages of BiFC such as convenience and direct visualization of PPI in ceils. However, BiFC has one common limitation： the separated non-fluorescent fragments can be spontaneously self-assembled into an intact protein, which leads to false-positive results. In this study, a pair of complementary fragments （sfGFPN and sfGFPC） was constructed by splitting superfolder GFP （sfGFP） between the 214 and 215 amino acid residue, and sfGFPC was mutated by site-directed gene mutagenesis to decrease the signal of negative control. Our results showed that mutations in sfGFPC （sfGFPC（ml2）） can effectively decrease the signal of negative control. Thus, we provide an improved BiFC tool for the analysis of PPI. Further, since the self-assembly problem is a common shortcoming for application of BiFC, our research provides a feasible strategy for other BiFC candidate proteins with the same problem.