中文摘要：

本试验旨在研究乳铁蛋白（lactoferrin,LF）对滇撒配套系断奶仔猪生产性能、血清抗氧化指标及组织抗氧化基因表达量的影响。选用（28±2）日龄滇撒配套系断奶仔猪192头,随机分为4个处理,每个处理6个重复,每个重复8头,对照组饲喂基础饲粮,LF1、LF2和LF3 3个试验组在基础饲粮基础上分别添加125、250和500mg/kg的LF。饲喂42d后,测定平均日增重（ADG）、平均日采食量（ADFI）、料重比（F/G）和腹泻率。采用试剂盒检测血清总抗氧化能力（T-AOC）、总超氧化物歧化酶（T-SOD）活力、丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSHPx）活力,采用实时荧光定量PCR（RT-qPCR）法测定心脏和肝脏谷氧还蛋白1（GRX1）、硫氧还蛋白1（TRX1）、超氧化物歧化酶（SOD）及GSH-Px基因表达量。结果表明：1）与对照组相比,LF2和LF3组显著提高了ADG、ADFI（P〈0.05）,LF1和LF2组显著降低了料重比（P〈0.05）,LF2和LF3组显著降低了仔猪的腹泻率（P〈0.05）。2）血清中,与对照组相比,LF1（P〈0.05）、LF2（P〈0.01）和LF3组（P〈0.05）T-SOD活力均显著或极显著提高;LF2组GSH-Px活力及T-AOC均显著提高（P〈0.05）;LF2和LF3组的MDA含量均显著降低（P〈0.05）。3）与对照组相比,LF1和LF2组GRX1、SOD和GSH-Px基因在心脏和肝脏中的表达量均增加,其中LF1组肝脏GSH-Px差异显著（P〈0.05）,LF2组肝脏GRX1、SOD和GSH-Px差异极显著（P〈0.01）;LF1组TRX1基因在肝脏中的表达量极显著提高（P〈0.01）;LF3组GRX1、TRX1基因在肝脏和心脏中及SOD、GSH-Px基因在心脏中的表达量均降低,其中心脏GRX1、TRX1和SOD差异极显著（P〈0.01）。结果提示：在断奶仔猪饲粮中添加一定量的LF（本试验添加250mg/kg最佳）可提高断奶仔猪的生长性能,提高血清抗氧化指标及心脏和肝脏中抗氧化基因GRX1、TRX1、SOD和GSH-Px的表达量。

英文摘要：

This experiment was conducted to investigate the effects of lactofferin （LF） on growth performance, serum antioxidant indices and tissue antioxidant gene expressions in Diansa weaned piglets. A total of 192 Diansa weaned piglets at the age of （28±2） days were randomly divided into 4 groups with 6 replicates in every group and with 8 piglets in every replicate. The control group was fed a basal diet, and LF1, LF2 and LF3 groups were respectively supplemented with 125, 250 and 500 mg/kg LF in the basal diet. Average daily weight （ADG） , average daily feed intake （ADFI）, feed to gain ratio （F/G） and diarrhea rate were determined after feeding 42 days. Kits were used for detecting serum antioxidant indices： total antioxidant capacity （T-AOC）, total superoxide dismutase （T-SOD） activity, malondialdehyde （MDA） content and glutathione peroxidase （GSH-Px） activity. The expression levels of glutraredoxin 1 （ GRX1 ） , thioredoxin 1 （ TRX1 ） , superoxide dismutase （SOD） and GSH-Px genes in heart and liver were detected by real-time quantitative PCR （RT-qPCR）. The results showed as follows： 1 ） compared with control group, LF2 and LF3 groups significantly improved ADG and ADFI （ P〈0.05）, LF1 and LF2 groups significantly （P〈0.05） reduced F/G, and LF2 and LF3 groups significantly reduced dairrhea rate （P 〈 0.05 ）. 2 ） In serum, compared with control group, T-SOD activity were significantly increased in LF1 （P〈0.05）, LF2 （P〈0.01） and LF3 （P〈0.05） groups, GSH-Px activity and T-AOC in LF2 group were significantly increased （P〈0.05）, and the contents of MDA in LF2 and LF3 groups were significantly decreased （P〈0.05）. 3） Compared with control group, the expression levels of GRX1, SOD and GSH-Px genes in heart and liver were increased in LF1 and LF2 groups. In liver, the difference of GSH-Px gene in LF1 group was significant （P〈0.05）, and those of GRX1, SOD and GSH-Px genes in LF2 group were extremely significant （P