中文摘要：

为了解楚雄地区猪伪狂犬病病毒（PRV）野毒感染状况,通过gE-ELISA方法对采集自楚雄地区2009年-2013年间累计2 811份血清进行了PRV野毒感染抗体检测,对4个规模养殖场猪场采取了疫病净化控制措施。共计检测场（户）数758户,总阳性率为31.70%;其中商品猪阳性率为26.34%,种猪阳性率为35.06%;规模场样本阳性率为37.67%;散养农户样本阳性率为28.62%;没有接种疫苗的母猪阳性率（56.82%）高于每年分别免疫接种1次（45.16%）、免疫接种2次（29.41%）和免疫接种3次（18.33%）的母猪阳性率。采取控制措施12个月后,4个试验猪场相对于对照猪场阳性率均明显下降。提示了猪伪狂犬病病毒普遍存在于楚雄地区养猪场,完善生物安全措施,制定合理的防疫制度,使用基因缺失疫苗配合检测、淘汰阳性猪是规模猪场控制该病进一步扩散的有效方式。

英文摘要：

In order to understand the infection status of PRV wild isolate in the farms located in Chuxiong region from 2009 to 2013,the PRV serum antibodies of 2 811 serum samples from different batches collected from different scale immunized farms of Chuxiong regain were detected by PRV gE-ELISA antibody detection kit.Prevention and control measures were taked in 4farms.The serological survey result showed that 758 farms were detected and the positive rate was 31.70%.The average positive rates of commercial pigs and boars were 26.34%and 35.06%respectively.The positive rates of scale farms and scattered farms were 37.67% and 28.62% respectively.The positive rate of PR in sows were 56.82%（un-vaccinated）,45.16%（once vaccinated）,29.41%（twice vaccinated）and 18.33%（three vaccinated）.The positive rate of PR in un-vaccinated sows were higher than the rate of once vaccinated,twice vaccinated and three vaccinated.After taking the prevention and control measures 12 months,the positive rate of PR apparently declined.The result showed that the infection of PRV wild isolate were widespread in Chuxiong region.Enhancing bio-safety,developing reasonable anti-epidemic systems and using by gene-deleted vaccine are efficient ways to control PR further spreading in farms.