中文摘要：

背景：白藜芦醇是一种广泛存在于葡萄、花生和多种药用植物中的多酚类化合物。现已明确白藜芦醇具有多种牛物活性，如抗肿瘤，抗炎等。许多证据表明白藜芦醇具有防治心血管疾病的作用，但其机制并不明确。 目的：观察白藜芦醇对大鼠急性心肌缺血的保护作用及其作用机制。 设计：随机分组平行对照实验。 单位：哈尔滨医科大学药学院药理教研室和黑龙江省生物医药莺点实验室。 材料：实验于2005—03／07在哈尔滨医科夫学药学院完成。选用健康成年Wistar大鼠60只，体质蕈250-300g。随机将大鼠分为5组：假手术组，空白对照组，白藜芦醇低、中、高剂量组（5，15，45mg／kg），每组12只。 方法：结扎大鼠左冠状动脉前降支建立急性心肌缺血模型。假手术组：只穿线不结扎。白藜芦醇组：按5，15，45mg／kg剂量静脉注射白藜芦醇（购自湖南华光生物制品有限公司，生产批号：20050221，纯度／〉99％）10min后结扎。模型组：静脉注射等量生理盐水10min后结扎。分别观察大鼠左冠状动脉前降支结扎前投结扎后1，5，10，15，30min标准Ⅱ导联心电图ST段的变化情况。心肌缺血6h后进行心肌红四氯唑染色确定心肌梗死面积；紫外分光光度法测定血清肌酸激酶和乳酸脱氢酶活力，确定心肌损伤程度。采用原位末端脱氧核糖核酸转移酶介导的d-UTP缺口末端标记法检测心肌细胞凋亡指数；采用链霉索抗生物素蛋白-过氧化物酶连接法检测凋亡相关基因Fas．Bcl-2及Bax的蛋白表达情况。计量资料差异比较采用t检验。 主要观察指标：各组大鼠冠状动脉结扎后的ST段上移情况，血清肌酸激酶和乳酸脱氢酶活力，心肌细胞凋亡指数，凋亡相关基因Fas．Bcl-2及Bax的蛋白表达情况比较。 结果：大鼠60只均进入结果分析。①结扎后1，5，10min白藜芦醇中、高剂量组ST?

英文摘要：

BACKGROUND： Resveratrol is a naturally occurring phytoalexin present in grapes, peanut and some herbs. It has been demonstratod to produce a variety of biological actions, such as antieancer, antiinflammation. Accumulating line of evidence supported the view that resveratrol may exert protective effect on cardiovascular system. However, its protective mechanism is not completely understood. OBJECTIVE： To investigate the anti-isehemic effect and mechanism of resveratrol （Res） on acute myocardial infarction in rats. DESIGN： Randomly grouping paralleled control study. SETTING： Pharmacological Laboratory of Harbin Medical University, Biopharmaceutical Key Laboratory of Heilongjiang Province-Incubator of State Key Laboratory. MATERIALS： The experinlent was carried out in Department of Pharmacology of Harbin Medical University from March 2005 to July 2005. Totally 80 male Wistar rats weighting 250-300 g were selected in this study. Among them, 60 rats after operation successful modeling were randomly grouped into 5 groups： sham operation group, blank control group, resveratrol 5 mg/kg, 15 mg/kg and 45 mg/kg groups with 12 in each group. METHODS： Acute myocardial infarction （AMI） model was induced by ligation of the anterior branch of the left coronary artery in rat, Sham operation group： The same suture was put through bat not ligated. Resvera： trol group： Rats were injected with 5, 15 and 45 resveratrol mg/kg provided by Hunan Huaguang Biological Products Company Limited （batch number： 20050221, purity 999%） and ligated after 10 minutes. Model group： The same volume saline was injected for 10 minutes and then rats were ligated. Observe and record ST segment of standard limb lead Ⅱ electrocardiogram （ECG） after I, 5, 10, 15, 30 minutes after ligating the left anterior decending coronary artery, After 6-hour ischemia, the infarct size areas was identified with the myocardium by 2, 3, 5-triphenyltetrazolium chloride （TTC） stain; the activities of serum crcatine kina