欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
由于HERG钾通道在心律失常的发生与治疗中具有双重作用，因此成为近年来研究的热点。本文主要通过免疫荧光化学染色法、激光共聚焦显微镜及Western blotting法分别定性、定量地检测不同浓度的苦参碱、氧化苦参碱和白藜芦醇对稳定转染在HEK-293细胞中HERG钾通道表达的影响。研究发现，苦参碱（1μmol·L^-1）和氧化苦参碱（1μmol·L^-1）均能够促进定位于HERG-HEK细胞膜上的HERG钾通道的表达（n=5，P〈0．05），苦参碱（100μmol·L^-1）能够抑制定位于HERG—HEK细胞膜上的HERG钾通道表达（n=5，P〈0．05），氧化苦参碱（100μmol·L^-1）对HERG钾通道表达没有影响，白藜芦醇不影响HERG-HEK细胞中HERG钾通道表达。结果表明，低浓度的苦参碱促进HERG钾通道表达，高浓度的苦参碱抑制HERG钾通道表达，低浓度的氧化苦参碱促进HERG钾通道表达，白藜芦醇不影响HERG钾通道表达。这为苦参碱、氧化苦参碱和白藜芦醇抗心律失常作用机制及安全性提供了理论依据。
英文摘要：
Because HERG potassium channel has important effects on both proarrhythmia and antiarrhythmia, we use immunofluorescence and Western blotting methods to detect the expression of HERG channel of HERG-HEK cells in different concentrations of matrine, oxymatrine and resveratrol. The findings showed that both matrine （ 1 μmol·L^-1 ） and oxymatrine （ 1 μmol·L^-1 ） increased HERG channel expression （ n = 5, P 〈 0.05 ） , while matrine （ 100 μmol ·L^-1 ） decreased HERG channel expression （ n = 5, P 〈 0.05 ） , resveratrol didn ＇ t, affect HERG channel expression. In conclusion, different concentrations of matrine and oxymatrine affect HERG channel expression, while there is no relationship between resveratrol and HERG channel expression. It provides a theoretical support for the safety and mechanism of anti-arrhythmic drugs.
同期刊论文项目
同项目期刊论文
Blockade of HERG K+ channel by isoquinoline alkaloid neferine in the stable transfected HEK293 cells
Hypoglycaemic and hypolipidaemic effects of emodin and its effect on L-type calcium channels in dysl
The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting H
Cardioprotective Effects and Underlying Mechanisms of Oxymatrine Against Ischemic Myocardial Injurie
Sphingolipid Metabolite Ceramide Causes Metabolic Perturbation Contributing to HERG K+ Channel Dysfu
Large-conductance Ca2+-activated K+ currents blocke and impaired by homocysteine in human and rat me
Ischemia impairs the association between connexin 43 and M3 subtype of acetylcholine muscarinic rece
Large-conductance Ca2+-activated K+ currents blocked and impaired by homocysteine in human and rat m
Modulation of HERG K+ channel expression by the naturally-occurring antiarrhythmics matrine and oxym
Indapamide induces apoptosis of GH3 pituitary cells independently of its inhibition of voltage-depen
JAK/STAT and PI3K/AKT Pathways Form A Mutual Transactivation Loop and Afford Resistance to Oxidative
位置：