中文摘要：

目的研究反复惊厥新生大鼠大脑皮质中自噬相关基因微管相关蛋白1轻链3（LC3）的表达及Cathepsin B抑制剂（CBI）对其表达的干预作用。方法 6日龄SD大鼠随机分为3组,每组30只。惊厥组新生大鼠每日吸入三氟乙醚诱导惊厥发作5次,每次间隔30 min,连续9 d;对照组同样操作,但不吸入三氟乙醚;CBI组惊厥前腹腔注射CBI（总量2μL）,采用同样方法吸入三氟乙醚诱导惊厥。3组大鼠于最后一次惊厥后分别按1.5 h、3.0 h、6.0 h、24.0 h和出生35 d（P35）取脑,采用Western blot法分别检测3组大鼠大脑皮质中LC3的表达。结果惊厥组（1.5 h、3.0 h、6.0 h、24.0 h）LC3的表达明显高于对照组同一时间点（Pa〈0.05）。CBI组LC3于1.5 h、3.0 h、6.0 h、24.0 h的表达明显低于惊厥组同一时间点（Pa〈0.05）。P35时间点3组间LC3水平比较差异无统计学意义。结论 LC3蛋白水平明显上调,表明新生大鼠惊厥后急性期自噬途径被激活,CBI参与了自噬途径的调控。

英文摘要：

Objective To explore the expression of microtubule-associated protein 1 light chain 3（LC3） in neonatal rat cerebral cortex after recurrent seizures and the intervention effect of Cathepsin-B inhibitor（CBI）.Methods Six-day-old（P6） SD rats were randomly divided into 3 groups： recurrent seizure group（RS group,n=30）,CBI-treated seizure group（CBI group,n=30） and control group（n=30）.Rats in RS group were subjected to 5 seizures with flurothyl for 9 d,at intervals of 30 min.Rats in control group without flurotlyl.In CBI group,CBI was injected each day before seizures were induced,and with flurothyl in order to induce seizures with the same way.Western blot was employed to determine LC3 expression at different time points（1.5 h,3.0 h,6.0 h,24.0 h） and P35 after the last convulsion.Results LC3 expressions in RS group（1.5 h,3.0 h,6.0 h and 24.0 h）were significantly higher than those at the same time in control group（Pa0.05）.LC3 expression in the time point（1.5 h、3.0 h、6.0 h and 24.0 h）of CBI group were significantly decreased compared with those in RS group（Pa0.05）.There were no significant differences among 3 groups at P35.Conclusions Autophagy/lysosomal pathway were activated immediately after recurrent seizures as indicated by the elevated expression of LC3 in cerebral cortex.CBI was involved in the regulation of autophagy/lysosomal pathway by down-regulating the acute phase expression of LC3.