中文摘要：

在接受者和施主的变化的背景分析造血的房间起源是极其有用的由施主淋巴细胞注入监视造血的干细胞移植(HSCT ) 和顺序的采纳免疫疗法的效果。我们基于顺序多型性系统开发了一个敏感、可靠、快速的即时 PCR 方法到份量上在 HSCT 以后估计造血的 chimerism。29 选择顺序多型性(SP ) 的方法 A 面板标记被即时 PCR 与白血病和另外的 hematological 疾病在 101 个 HSCT 病人屏蔽。在宽恕和恶化状况的 8 个 HSCT 病人的骨头髓样品的 chimerism 动力学是跟随的纵。结果接受者遗传型辨别在 97.0% 是可能的(101 中的 98 个) 与 2.5 的一个吝啬的数字(1-7 ) ，增进知识的标记每接受者 / 施主配对。用包含特定的 SP 标记的 plasmids 的连续冲淡， 0.99 的线性关联(r) ，在 -3.2 和 -3.7 和 0.1% 的敏感之间的斜坡被证明可再现。由这个方法，它对可能很精确地从 0.1% ～ 30% 在范围检测自体同源的信号。在低于 5% 的自体同源的信号的重要范围的方法的精确性非常地高(标准差 <1.85%) ，它可能显著地在后续的移植以后的功课早在自体同源的信号改进变化的察觉精确性。在短双人脚踏车重复 PCR chimerism 试金上的即时 PCR 方法的主要优点是 PCR 竞争和高原偏爱的缺席，与表明的更大的敏感和线性。最后，我们有希望地分析了收到了 allografts 并且介绍了说明了敏感水平和这个方法的有希望的临床的申请的宽恕和恶化状况的 chimerism 动力学的 8 个病人的骨头髓样品。这基于 SP 的即时 PCR 试金提供对能在预言接枝拒绝和早恶化有用的混合 chimerism 的一个快速、敏感、精确的量的评价的结论。

英文摘要：

Background Analysis of changes in recipient and donor hematopoietic cell origin is extremely useful to monitor the effect of hematopoietic stem cell transplantation （HSCT） and sequential adoptive immunotherapy by donor lymphocyte infusions. We developed a sensitive, reliable and rapid real-time PCR method based on sequence polymorphism systems to quantitatively assess the hematopoietic chimerism after HSCT. Methods A panel of 29 selected sequence polymorphism （SP） markers was screened by real-time PCR in 101 HSCT patients with leukemia and other hematological diseases. The chimerism kinetics of bone marrow samples of 8 HSCT patients in remission and relapse situations were followed longitudinally. Results Recipient genotype discrimination was possible in 97.0% （98 of 101） with a mean number of 2.5 （1-7） informative markers per recipient/donor pair. Using serial dilutions of plasmids containing specific SP markers, the linear correlation （r） of 0.99, the slope between -3.2 and -3.7 and the sensitivity of 0.1% were proved reproducible. By this method, it was possible to very accurately detect autologous signals in the range from 0.1% to 30%. The accuracy of the method in the very important range of autologous signals below 5% was extraordinarily high （standard deviation 〈1.85%） which might significantly improve detection accuracy of changes in autologous signals early in the post-transplantation course of follow-up. The main advantage of the real-time PCR method over short tandem repeat PCR chimerism assays is the absence of PCR competition and plateau biases, with demonstrated greater sensitivity and linearity. Finally, we prospectively analyzed bone marrow samples of 8 patients who received allografts and presented the chimerism kinetics of remission and relapse situations that illustrated the sensitivity level and the promising clinical application of this method. Conclusion This SP-based real-time PCR assay provides a rapid, sensitive, and accurate quantitative assessment of mixed chim