中文摘要：

目的：构建MAGE-1（melanoma antigen 1）与人HSP70（heat shock protein70）融合基因的真核表达载体pCDNA3．1-MAGE-1-HSP70，为肿瘤DNA疫苗研究奠定基础。方法：利用PCR方法扩增人HSP70基因，经测序后连入真核表达载体pCDNA3．1，再通过PCR方法扩增MAGE-1基因，测序后插入HSP70基因的5’端，构建了MAGE-1与人HSPTO融合基因表达载体pCDNA3．1-MAGE-1-HSP70。结果：成功地扩增了人HSP70基因与MAGE-1基因，测序结果表明与GenBank公布的序列一致，成功地构建了pCDNA3．1-MAGE-1-HSP70真核表达载体。结论：成功地构建MAGE-1与人HSP70融合基因的真核表达载体pCDNA3．1-MAGE-1-HSP70，为进一步DNA肿瘤疫苗研究提供了实验基础。

英文摘要：

Objective：To construct the MAGE - 1 and human HSP70 fusion gene eukaryotic expression vector Methods ：The HSP70 and MAGE - 1 genes were amplified by PCR. After sequencing, the two genes were cloned into the eukaryotic expression vector pCDNA3.1 to construct fusion gene expression plasmid pCDNA3.1 - MAGE - 1 - HSP70. Results：The HSP70 gene and MAGE - 1 gene were amplified successfully from plasmids, and their sequences were identical with that reported in GenBank. The fusion gene expression plasmid pCDNA3.1 - MAGE - 1 - HSP70 was successfully constructed. Conclusion ：The MAGE -1 and Hsp70 fusion gene eukaryotic expression vector pCDNA3.1 - MAGE - 1 - HSP70 was constructed, which prepared the materials for the research of DNA vaccine.