中文摘要：

目的：构建MAGE-1（melanoma antigen 1）与人HSP70（heat shock protein 70）融合基因的真核表达载体PIRES2-EGFP-MAGE-1-HSP70,并观察在小鼠黑色素瘤细胞内的表达,为肿瘤DNA疫苗研究奠定基础.方法：HSP70基因经酶切后连入真核表达载体PIRES2-EGFP,再通过PCR方法扩增MAGE-1基因,测序后插入PIRES2-EGFP-HSP70中HSP70基因的5端,构建了MAGE-1与人HSP70融合基因表达载体PIRES2-EGFP-MAGE-1-HSP70.结果：扩增了MAGE-1基因,测序结果表明与GenBank公布的序列一致,成功地构建了PIRES2-EGFP-MAGE-1-HSP70真核表达载体.结论：成功地构建MAGE-1与人HSP70融合基因的真核表达载体PIRES2-EGFP-MAGE-1-HSP70,并在小鼠黑色素瘤细胞系稳定表达,为进一步DNA肿瘤疫苗研究提供了实验基础.

英文摘要：

Objective：To construct the MAGE - 1 and human HSP70 fusion gene eukaryotic expression vector and the expression in mouse melanoma cells in vitro. Methods： MAGE - 1 genes were amplified by PCR. After sequen cing, the two genes were cloned into the eukaryotic expression vector PIRES2 - EGFP to construct fusion gene expres sion plnsmid PIRES2 - EGFP - MAGE - 1 - HSP70. Results： The MAGE - 1 gene were amplified successfully from plasmids, and their sequences were identical with that reported in GenBank. The fusion gene expression plasmid PIRES2 - EGFP - MAGE - 1 - HSP70 was successfully constructed. Conclusion： The MAGE - 1 and Hsp70 fusion gene eukaryotic expression vector PIRES2 - EGFP - MAGE - 1 - HSP70 was constructed which prepared the materials for the research of DNA vaccine .