中文摘要：

目的：探讨MAGE-3，MAGE-n抗原表位体外联合诱导的CTL，并研究其特异性杀伤活性。方法：候选抗原表位以固相多肽合成技术合成，并用HPLC进行纯化，质谱法（Ms）鉴定，以流式细胞仪筛选HLA-A2^＋人外周血PBMC，T2细胞负载抗原肽反复刺激活化诱导抗原特异性CTL，LDH检测其杀伤活性。结果：联合表位肽体外刺激人PBMC，能够较强地诱导抗原特异性CTL并产生特异性杀伤。结论：MAGE-3与MAGE-n的HLA-A2限制性CTL表位肽的联合应用能够产生较强的体外抗肿瘤免疫反应。

英文摘要：

Objective ：To explore peptide-specific CTLs response and the immunological activity of the HLA- A2-restricted CTL candidate epitopes derived from tumor antigen MAGE-3 and MAGE-n in vitro. Methods： The MAGE-3 and MAGE-n candidate HLA-A2 restricted CTL epitopes were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Peptide-specific CTLs were induced from the peripheral blood mononuclear cells （PBMC） of HLA-A2 positive healthy donors by multiple stimulations with peptide-pulsed T2 cells. The capability of CTLs recognizing and lysing HLA-A2^＋ MAGE- 3^＋ and MAGE- n ＋ tumor cells was detected with lacatate dehydrogenase （LDH） release assay. Results： The results demonstrated the combination of MAGE -3 and MAGE-n epitope tumor-specific CTL responsed in vitro specifically and effectively. Conclusion： Combination of two epitopes could elicit stronger MAGE-specific antitumor immunity.