中文摘要：

为获得尤文肉瘤EWS-FLI1融合蛋白有效的HLA-A2.1限制性CTL表位,综合运用BIMAS,SYFPEITHI,Predep和IEDB方案对EWS-FLI1进行CTL表位的预测,得出8个CTL表位9肽序列;应用多项式方案、量化基序方案对8个表位9肽进一步进行筛选,获得其中4个9肽序列,并进一步运用分子模拟方法初步模拟了4个表位肽与HLA-A2.1分子间的相互结合作用;应用标准Fmoc方案合成CTL表位肽,RP-HPLC与MS分别鉴定合成肽的纯度与分子量;通过亲和力实验验证了表位肽QIQLWQFLL（EWS-FLI1304）与HLA-A2.1有较强的结合力,从而为表位肽的后继免疫学特性研究提供了基础.本研究提供了一个合理高效的表位筛选方法.

英文摘要：

To obtain the HLA-A2.1-restricted CTL epitopes of EWS-FLI1 fusion protein of Ewing＇s sar- coma, EWS-FLI1 protein was synthetically predicted by BIMAS, SYFPEITHI, Predep and IEDB methods. Eight epitopes predicted by above methods were further screened by the polynomial method and the quantitative motif method as well. Four screened peptides were stimulated to dock with HLA-A2.1 by molecular dynamics simulation. All peptides were synthesized with standard Fmoc strategy, then detected beyond 98% in purity by reverse phase high performance liquid chromatography （RP-HPLC） and measured the values of molecular weight of them by mass spectrometry （MS）. Infinity experiment validated the infinity of peptide QIQLWQFLL（Ews-FLI1 304）with HLA-A2.1 molecule, which could afford the foundation of immunological characteristics study of this screened peptide. This research provides a rational and high efficient method to screen epitope.