中文摘要：

GsCRCK基因是参与胁迫早期应答的钙/钙调素调控的受体类蛋白激酶基因,研究发现GsCRCK正向调控拟南芥对NaCl和ABA胁迫的耐性,将耐盐蛋白激酶基因GsCRCK转化苜蓿,对于增强苜蓿的耐盐性具有重要的现实意义。本研究采用农杆菌介导法将其转入农菁1号苜蓿,获得大量抗性植株。经PCR和RT-PCR检测证明GsCRCK基因已整合到农菁1号苜蓿基因组中并在转基因植株中转录表达。对获得的2个转基因株系进行耐盐性分析,在300mmol/L NaCl条件下进行胁迫处理,测定处理0,3,6,9,12,15d后的质膜透性、丙二醛（MDA）和叶绿素（Chl）含量,以及胁迫15d时的SOD活性;并统计400mmol/L NaCl处理15d时各株系的死亡率。结果显示,300mmol/L高盐胁迫15d后转基因苜蓿仍能正常生长,而野生型苜蓿则遭受盐害严重;转基因苜蓿的相对电导率极显著低于野生型,MDA含量也显著低于野生型,而Chl含量和SOD活性都显著高于野生型;在400mmol/LNaCl处理下,2个转基因株系的死亡率分别为13.33%和10.00%,明显低于野生型植株（63.33%）。表明GsCRCK基因的导入提高了转基因苜蓿的耐盐性。

英文摘要：

The stress-responsive kinase gene of wild soybean（GsCRCK） was selected because of the gene expression profiles under salinity,drought and cold stresses,previously established in our laboratory.Over expression of GsCRCK in transgenic Arabidopsis resulted in enhanced plant tolerance to high salinity and ABA.In this study,one plant expression vector regulated by a 35S promoter（named as pBEOCRCK） were constructed to transfer the target gene into Medicago sativa cv.Nongjing No.1 separately by the Agrobacterium-mediated method.Many resistant seedlings of GsCRCK have been obtained.The results of PCR and RT-PCR showed that the GsCRCK gene was normally expressed in transgenic plants.After GsCRCK transgenic M.sativa cv.Nongjing No.1 were stressed with 0,300 and 400 mmol/L NaCl,we compared transgenic M.sativa to non-transgenic M.sativa by assessing specific physiological indicators.The relative membrane permeability,the content of chlorophyll,the content of MDA,and the activity of SOD in plants which were stressed with 300 mmol/L NaCl were measured together with the death rate from a 400 mmol/L NaCl stress.The transgenic M.sativa had a greater tolerance of salinity stress compared with non-transgenic plants.