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中文摘要：
从野生大豆中获得的具有耐旱、耐冷特性的锌指转录因子GsZFP1基因,构建到以CaMV35S为启动子、E12为增强子的植物表达载体pCEOM中,以bar基因为筛选标记基因,通过农杆菌介导法将构建的植物表达载体转化苜蓿品种农菁1号的子叶节,用含0.5mg/L草丁磷的筛选培养基进行筛选,获得了70株抗性植株,用PCR检测得到20株bar基因阳性植株,将获得的转基因植株进行GsZFP1基因的RT-PCR鉴定,获得3株RT-PCR阳性植株。结果表明,GsZFP1基因在苜蓿中得到表达。
英文摘要：
GsZFP1 gene is a zinc finger transcription factor got from wild soybean, which is resistant to drought and tolerant to cold. GsZFP1 gene was built into CaMV35S promoter and El2 enhanser into the plant express vec- tor pCEOM. The selectable marker gene was bar gene. Medicago sative was transformed using Agrobacterium turne- faciers and resistant plants, followed by selective screening and PCR, RT-PCR analysis. The results showed that the GsZFP1 was expressed in M. sativa. L.
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