中文摘要：

目的：旨在观察离心运动致骨骼肌损伤后丝氨酸蛋白酶（Omi）在骨骼肌组织中的表达变化。方法：8周龄雄性SD大鼠36只,随机分为安静对照（n=6）、离心运动2大组（n=30）,离心运动组大鼠在跑台上进行一次性离心运动,分别于运动后即刻（E0组,n=6）、运动后12 h（E12组,n=6）、运动后24 h（E24组,n=6）、运动后48 h（E48组,n=6）和运动后72 h（E72组,n=6）麻醉大鼠,腹主动脉取血,取比目鱼肌待测;使用荧光酶联免疫吸附法检测半胱氨酸天冬氨酸特异性蛋白酶-3（Caspase-3）的活性;Western Blot和Realtime-PCR法分别检测Omi、凋亡抑制蛋白（XIAP）的蛋白表达和基因水平;Co-IP法检测XIAP和Omi的相互结合作用。结果：与对照组相比,离心运动后Caspase-3、Caspase-9活性均明显升高;离心运动后Omi、XIAP的蛋白表达和m RNA水平均明显升高;离心运动能明显增加XIAP和Omi的结合量。结论：离心运动能够激活骨骼肌组织Caspase-9和Caspase-3的活性,诱导Omi和XIAP的表达上调,促进XIAP与Omi相互结合,Omi可能在促使凋亡过程中发挥重要介导作用。

英文摘要：

Objective：The aim of the present study is to investigate the change of Omi in skeletal tissue injury after eccentric exercise and the underlyingmechanism. Methods：36 Adult SD rats,8 weeks aged,were randomly divided into two groups：control group and five kinds exercise groups which included 0 hafter exercise,12 h after exercise,24 h after exercise,48 h after exercise and 72 h after exercise. The exercise groups were made to run on amotor-driven forone-off eccentric exercise. The rats were sacrificed in batches at different time points after exercise,then the soleues were saved to measure. Caspase-3and-9 were determined by flurometric immunosorbent enzyme assay method. Protein and m RNA levels of Omi,XIAP were determined by Western blot andReal time PCR,respectively. The combination of XIAP and Omi was determined by Co-IP. Results：Compared with those in control group,Caspase-3and-9 activity were significantly increased after eccentric exercise;Protein expression and m RNA levels of Omi,XIAP were significantly increased after ec-centric exercise. Eccentric exercise improved the combination of XIAP and Omi. Conclusions：Eccentric exercise can increase the expression of Omi andXIAP,improve the combination of XIAP and Omi,induce enhanced Caspase-9 and-3 activity and then induce skeletal muscle cell apoptosis.