中文摘要：

以玻碳电极（GCE）为基底，采用恒电位法沉积一层普鲁士蓝（PB），然后将j芑四甲酸二酐衍生物（PTC-NH，）自组装到其表面，形成既带氨基功能团，又可有效防止PB渗漏的导电膜。通过静电吸附和共价键合作用固定纳米金和辣根过氧化物酶（HRP）的复合物，从而制得性能优良的过氧化氢（H2O2）生物传感器。采用循环伏安法（CV）和计时电流法，考察了传感器的电化学性能。实验表明，本传感器具有灵敏度高、线性范围宽、检出限低、稳定性好、抗干扰能力强等特点。其线性范围为2．0×10^-6～1．4×10^-5mol／L；检出限为8．3×10^-7mol／L（S／N=3）。

英文摘要：

A novel type of hydrogen peroxide biosensor was prepared. First, a stable Prussian Blue （PB） film was electrodeposited on glassy carbon electrode by applying constant potential. After that, the derivative of 3,4,9,10-perylenetetracarboxylic dianhydride containing amino group was immobilized on the surface of PB by self-assembly, which can effectively prevent the leakage of PB. Then the composite of nano-gold and horseradish peroxidase （HRP-Au） was successful immobilized onto the above prepared film to construct the hydrogen peroxide biosensor via the effect of electrostatic adsorption and process of covalent bonding. The per- formance of the biosensor was characterized by cyclic voltammetry and the chronoamperometry. The proposed biosensor exhibited high sensitivity, broader linear range, a lower detection, excellent stability as well as good selectivity for H202 determination. And the linear range was from 2.0×10^-6～1.4×10^-5mol/L with a detection limit of 8.3 ×10^-7 mol/L（ S/N = 3）.