中文摘要：

将DMF（N,N-二甲基甲酰胺）分散的多壁碳纳米管（MWNT）修饰在金电极表面,再将修饰电极依次沉积纳米金和L-半胱氨酸（L-Cys）,并通过半胱氨酸中的巯基吸附Fe3O4@Au纳米复合材料,再固载甲胎蛋白抗体（anti-AFP）,以牛血清白蛋白（BSA）封闭非特异性吸附位点,构建了高灵敏、稳定的新型电流型甲胎蛋白免疫传感器。实验通过扫描透射电子显微镜（TEM）对DMF-MWNT和Fe3O4@Au复合纳米粒子进行了表征。在优化的实验条件下,此免疫传感器对甲胎蛋白抗原的检测范围为0.1～150μg/L,检出限为0.03μg/L。

英文摘要：

N,N-Dimethylfomamide（DMF） dispersed MWNTS was firstly modified on the electrode surface,which was in further eletrodeposited a nano-Au layer for immobilization of L-cysteine.Subsequently,Fe3O4@Au nanocomposites were absorbed on the modified electrode surface by the SH-Au bond for increasing the specific surface area and further immobilizing anti-AFP.Finally,bovine serum albumin（BSA） was used to block the non-specific adsorption sites of the immunosensor to obtain a highly sensitive and stable immunosensor.Experiments by transmission electron micros-copy（TEM） were carried out to characterize the prepared MWCNTs and Fe3O4@Au composite nanoparticles.Under optimal conditions,the sensor has a good response to AFP,in the detection range form 0.1 to 150 μg/L,and detection limit of 0.03 ng/mL.