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中文摘要：
目的：建立一种从巴豆中提取纯化及制备巴豆苷的方法。方法：以巴豆苷得率为指标，采用正交试验设计优化巴豆苷的提取工艺参数；考察大孔树吸附脂纯化工艺条件；应用制备型液相色谱分离高纯度巴豆苷。结果：最佳巴豆苷提取工艺参数为8倍量20％乙醇提取3次，每次40min；大孔树脂吸附初步纯化后，制备液相分离出巴豆苷单体，质量分数达98％，可作为对照品。结论：该方法简便易行，可用于分离制备高纯度巴豆苷样品。
英文摘要：
Objective： To establish a method for extracting, purifying and preparing isoguanosine from Croton tiglium. Method： Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result： Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol, 40 min each time. After preliminarily purifying by macroporous resin, isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion： This method was simple and easy to operate, also could be used for preprartion high purity isoguanosine samples.
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