中文摘要：

目的：探讨寻常型银屑病患者外周血外周血单一核细胞（PBMCs）Toll样受体9（Toll—like receptor9，TLR9）的表达及其与血清中IFN-γ浓度的相关性，初步探讨TLR9在寻常型银屑病发病中的作用。方法：采用荧光定量PCR技术检测外周血单个核细胞TLR9的表达，并采用双抗体夹心酶联免疫吸附法（ELISA）检测血清IFN-γ的浓度。另取20例正常人作为对照组．结果：寻常型银屑病患者外周血中单核细胞TLR9的表达较正常人对照组明显增高（P〈0．01），TLR9表达与血清IFN-γ浓度变化呈正相关（P〈0．05）。结论：结果提示TLR9及其介导的天然免疫鹰答在银屑病发病机制中起重要作用，其机制可能是通过上调IFN-γ的表达促使炎症因子产生和分泌而介导银屑病的发病。

英文摘要：

Objective：To explore the expression of TLR9 in human peripheral blood mononuclear cell and its correlation with concentration of IFN - γ in patient with psoriasis vulgaris. Methods：The TLR9mRNA in peripheral blood mononuclear cell were measured by Real -time quantitative PCR. The study group was composed of 30 cases of patient with psoriasis vulgaris and 20 normal persons as control. The level of IFN - γ in sera were measured by double antibody sandwich enzyme- linked immunosorbent assay （ELISA）. Results：The expression of TLR9 in human peripheral blood mononuclear cell and the serum level of IFN - γ were significant increased in patients with psoriasis vulgaris as compared with normal control （ P 〈 0.01 ） , and the expression of TLR9 was positively correlated with the concentration of IFN - γ. Conclusion ： The TLR9 and the innate immune response might play a critical role in the pathogenesis of psoriasis vulgaris, which may be through spurring the excretion of inflammatory factor.