中文摘要：

目的：设计特异性引物建立油茶白绢病齐整小核菌的快速分子检测体系。方法：扩增齐整小核菌核糖体DNA ITS区并测定其序列,比较该序列与GenBank中近似种的ITS序列差异,设计了特异性引物BF1和BR2。结果：该引物可以从齐整小核菌中扩增得到约540bp特异性条带,而扩增其它近似或相关菌株时没有相应的特异性条带。在25μL PCR反应体系中,引物BF1和BR2检测灵敏度为1pg浓度DNA。结论：利用设计的BF1和BR2特异性引物结合PCR方法可快速的扩增出齐整小核菌DNA,检测灵敏度为1pg.但在生产实践中诊断油茶白绢病发病前组织中的齐整小核菌还需要进一步研究。

英文摘要：

Objective：Two taxon-selective primers have developed for quick identification of the Athelia rolfsii.Method：These primers,BF1andBR2 were designed by comparing the aligned sequences of internal transcribed spacer regions（ITS） of a range of Fusarium species.Result：The primers showed good specificity for the Athelia rolfsii,and the approximately 540bp product was amplified exclusively.PCR sensitivity was 1 pg for genomic DNA extracted from Athelia rolfsii mycelium.No amplification products were detected with PCR of DNA from Fusarium genus using these primers.Conclusion：The assay is useful for rapid identification of Athelia rolfsii.The application of these PCR methods for early diagnosis of the seedling and Root Rot disease of Camellia oleifera needs to be studied further.