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中文摘要：
目的研究不同长度微电子机械系统（MEMS）微针条件下，雷公藤甲素（TP）不同剂型对大鼠类风湿性关节炎（RA）模型皮肤损伤的影响。方法制备TP丙二醇水溶液、脂质体、脂质体水凝胶贴剂3种剂型，采用Ⅱ型胶原诱导的大鼠RA模型，对大鼠左右侧皮肤分别作100和200仙mMEMS微针处理，用紫外分光光度法检测皮肤乳酸脱氢酶（LDH）活性。结果给予MEMS微针处理后，大鼠皮肤LDH活性没有显著变化（P〉0．05）；TP不同剂型均使皮肤LDH活性明显升高（P〈0．05），其中溶液组升高最大（P〈0．001），脂质体组次之（P〈0．01），脂质体水凝胶贴剂组升高最小（P〈0．05），但是左右两侧皮肤比较无明显差异（P〉0．05）。结论100和200岬MEMS微针均可安全地用于RA大鼠的经皮给药促透；TP制备为脂质体水凝胶贴剂后可显著地减小对RA大鼠皮肤的损伤，是其透皮给药的理想剂型。
英文摘要：
OBJECTIVE To study the effects of different triptolide formulations given by micro electro mechanical systems （MEMS） micro-needles on skin injury of rheumatoid arthritis rats . METHODS Three formulations of triptolide were prepared inclu- ding the aqueous solution of 20% propylene glycol, liposomes, and liposomes hydrogel patch. Rats with rheumatoid arthritis induced by collagen II were used, and 100 and 200 μm MEMS micro-needles treatment was performed on left and right sides of rat skin. The lactate dehydrogenase （LDH） activities were measured by ultraviolet spectro-photometric method. RESULTS After processed by MEMS micro-needle, the LDH activities of RA rat skin did not change obviously （ P 〉 0. 05 ）. All triptolide formulations increased LDH activities （ P 〈 0. 05 ）, and the increasement of solution group was largest （ P 〈 0. 001 ）, followed by lipsomes group （ P 〈 0. 01 ） and liposomes hydrogel patch （P 〈 0. 05）. Nevertheless, there was no significant difference between left and right sides of rat skin （P 〉 0. 05 ）. CONCLUSION Both 100 and 200 Ixm MEMS micro-needles were safe for enhancement of transdermal delivery in rheu- matoid arthritis rats. The skin injury was observably relieved when triptolide was prepared as liposomes hydrogel patch which is a per- fect formulation for transdermal delivery of triptoiide.
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