中文摘要：

采用化学共沉淀法结合高锰酸钾氧化制得表面羧基化的磁性微球,并吸附于Aeromonos sp.F3菌体表面,在磁场的作用下实现细胞分离。分别从菌/磁性微球相对质量比、pH、温度等方面对固定化菌体细胞的影响做了研究。Aeromonos sp.F3菌体的固定化最佳条件确定为,菌/磁性微球相对质量比为3.1,反应环境的pH和温度分别为8和25℃,固定化时间为0.5h。在磁场下分离并更换新鲜发酵培养液的条件下,该磁性固定化菌体可重复利用7次,其产生的胞外酶的相对活力仍保持在50%~60%。该方法可以实现Aeromonos sp.F3菌体细胞的磁场分离和重复利用。

英文摘要：

The carboxyl-functioned magnetic nanoparticle was prepared by the co-precipitation method with oxidized by KMnO4.The magnetic nanoparticle was directly adsorbed to the cell surface of Aeromonossp.F3.It was the new means to reutilization cells under the magnetic field.The optimum immobilization condition was that the cells were immobilized for 0.5hat 25 ℃with the cell/particle ratio of 3.1and the pH was 8.The residual enzyme activity was at 50%-60% after the immobilized cells were re-cultured for seven times in the fresh fermentation medium.