欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
改进青霉素 acylase (PA ) 的共有原子价固定，宏分子的拥挤理论被用于它的固定。到硅石的酶的集体比率的影响，以及，有在装配 PA 的活动的 glutaraldehyde 的激活时间，被学习。在中央毛孔，酶的固定上的β - 出租机动三轮车糊精(β - CD ) 的效果也被调查。当青霉素 acylase 在中央毛孔 covalently 集合了时，分别地，联合收益和相对活动到达了 99.5% 和 92.3% ，是显著的。这里的结果显示拥挤的那个模仿的大分子能显著地改善 covalently 使不能调动的 PA 的表演。
英文摘要：
To improve the covalent immobilization of penicillin acylase （PA）, macromolecular crowding theory was applied to its immobilization. Influence of mass ratio of enzyme to the silica, as well as, activation time with glutaraldehyde on the activity of assembled PA, was studied. In the mesopores, the effect of fl-cyclodextrin （β-CD） on the immobilization of the enzyme was also investigated. It was remarkable that the coupled yield and relative activity reached 99.5% and 92.3%, respectively, when penicillin acylase assembled covalently in the mesopores. The results here indicate that mimicked macromolecule crowding could significantly ameliorate the performance of covalently immobilized PA.
同期刊论文项目
同项目期刊论文
期刊信息
位置：