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中文摘要：
运用氧化还原反应在二氧化硅表面沉积组装了银纳米粒子，并在该载体表面共价连接木瓜蛋白醇，试图组合超微载体与化学共价固定化醇的优点，进一步提高组装后的醇活性。初步研究了微球尺寸及银纳米粒子对组装醇活力影响。实验结果表明，在给醇量为33．3mg／g时，木瓜蛋白醇直接共价连接在二氧化硅表面时活力仅为402u／mg，而在蛆装有银的载体上时活力为536u／mg，催化活性提高30％左右。同时在高给醇量条件下，醇活力回收提高了60％左右，活力回收最大值达到了30．5％，明显优于戊二醛直接共价固定的文献报道值。
英文摘要：
Silver nanoparficles formed by oxidation-reduction were deposited on the silica spheres and papain was covalently linked to the spheres, which was intended to combine the advantages of ultra-structure support and covalent immobilization to improve the activity of assembled papain. Effects of silver nanoparticles and diameter of silica spheres on the activity of papain were studied. The results showed that, the activity of papain was 402u/mg when coupled to bare silica spheres, but to silica spheres with deposition of silver nanoparticles it was 536u/mg, an increase by about 30%. The activity recovery of papain also increased by about 60% and maximum reached 30.5%, which was obviously better than that literature value in covalent immobilization with glutaraldehyde.
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