目的分析2008~2009年度中山地区3家综合医院临床分离的病原菌种类、构成比以及耐药性特点。方法收集临床送检各类培养标本,采用全自动细菌鉴定仪进行细菌鉴定,用NCCLS推荐的K-B法进行药物敏感试验,并对特殊耐药菌进行鉴定。结果各年度均以革兰阴性杆菌为主,2008~2009年度大肠埃希菌、铜绿假单胞菌、克雷伯菌属、鲍曼不动杆菌等常见革兰阴性杆菌对亚胺培南、阿米卡星、哌拉西林/他唑巴坦等抗菌药物的耐药率较低。金黄色葡萄球菌和肠球菌等革兰阳性球菌对万古霉素、利奈唑烷等具有高度的敏感性。结论 2008~2009年临床标本送检情况与病原菌分布基本一致,应提高临床标本送检率,加强实验室特殊耐药菌监测,合理使用抗生素,以减缓耐药株的蔓延,减少二重感染机会。
Objective To investigate the drug resistance of clinical isolates from 3 general hospitals in Zhangshan area from 2008 to 2009.Methods Various clinical cultures were collected,and automated bacterial identification system was used for bacteria identification.Drug susceptibility was tested by K-B method which recommended by NCCLS.The special resistant bacterial were identified.Results Gram negative bacilli were mainly caused respiratory tract or urinary tract infection.During 2008 to 2009,drug sensitivity tests showed that common gram-negative bacilli such as Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii and Pseudomonas aeruginosa were highly susceptive to imipenem,amikacin and piperacillin/tazobactam,Enterococcus faecalis.Staphylococcus aureus and Staphylococcus epidermidis were highly sensitive to Vancomycin and nitrofurantoin.Conclusion Clinical specimens was same to pathogens localities in 2008~2009.There was a high detection rate of fungi.The submission rate of clinical samples should be increased,the monitor of special resistant bacteria should be strengthened,the antibiotics should be rational applied to slow the spread of resistant strains,and reduce the opportunities of super infection.