中文摘要：

利用细胞免疫组化、原位杂交技术研究钙信号与缺氧诱发肺动脉平滑肌细胞（PASMC）癌基因c—los、c—myc表达的关系。结果显示，钙通道拮抗剂verapamil（V）、nifedipine（N）、Cyclosporin A（C）显著抑制缺氧引起的c-fos和f—myc mRNA的表达（其中c-fos mRNA：缺氧组198．1±32．8，缺氧＋V组161．6±24．1，缺氧＋N组173．7±35．8，缺氧＋C组159．0±32．8，P〈0．01；c-myc mRNA：缺氧组187．1±22．4，缺氧＋V组150．5±22．6，缺氧＋N组155．1±25．4，缺氧＋C组148．9±29．7，P〈0．01）。同时显著抑制缺氧引起的c—fos和c-myc蛋白的表达（其中c-los蛋白：缺氧组225．9±37．0，缺氧＋V组162．3±33．6，缺氧＋N组157．4±28．9，缺氧＋C组151．6±37．3，P〈0．01；c-myc蛋白：缺氧组228．8±33．4，缺氧＋V组170．2±26．7，缺氧＋N组170．7±28．5，缺氧＋C组179．1±29．2，P〈0．01）。verapamil和nifedipine能够显著抑制缺氧引起的癌基因表达，表明钙信号转导参与了缺氧引起PASMC癌基因的表达过程。Cyclosporin A可显著抑制缺氧引起的癌基因的转录表达，表明钙信号转导系统中的Ca^2＋／CaM—CaN转导通路在PASMC增殖过程中具有重要作用。PASMC增殖是肉鸡腹水综合征的主要特征，钙信号转导激活引起PASMC增殖的c—fos和c—myc的转录表达，表明钙信号转导可能与肉鸡腹水综合征的发生发展密切相关。

英文摘要：

This experiment aimed to study the relationship between calcium signal transduction and expression of c-fos,c-myc in broiler＇s Pulmonary Arterial Smooth Muscle Cell （PASMC） induced by hypoxia. The effect of calcium signal transduction on the oncogene c-fos and c-myc expression in PASMC induced by hypoxia was observed by immunohistochemistry, in situ hybrid- ization and image analysis methods. The results showed that the antagonism of calcium channel such as verapamil （V）, nifedipine（N） ,Cyclosporin A（C） can restrain such an expression （Expression level of c-fos mRNA was 198. 1±32.8 in hypoxia group, 161.6±24. 1 in hypoxia＋V group, 173.7±35.8 in hypoxia＋N group, 159.0±32.8 in hypoxia＋C group, P〈0.01; Expression level of c-myc mRNA was 187. 1±22.4 in hypoxia group, 150.5±22.6 in hypoxia＋V group, 155.1±25.4 in hypoxia＋N group, 148.9±29.7 in hypoxia＋C group, P〈0.01） and the antagonism can restrain c-fos and c-myc protein expression （Expression level of c-fos protein was 225.9±37.0 in hypoxia group, 162.3±33.6 in hypoxia＋V group, 157.4±28. 9 in hypoxia＋N group, 151.6±37.3 in hypoxia ＋ C group, P〈0.01 ; Expression level of e-mye protein was 228.8±33.4 in hypoxia group, 170. 2±26.7 in hypoxia＋V group, 170. 7±28.5 in hypoxia＋N group, 179. 1±29.2 in hypoxia＋C group, P〈0. 01）. PASMC proliferation were the important cause of aseites syndrome（AS）. Verapamil and nifedipine can significantly restrain the c-fos and c-myc expression of PASMC, indicating that calcium signal transduetion was important to the development of AS. Cyclosporin A can significantly restrain c-fos and c-myc expression of PASMC indicating that the Ca^2＋/CaM-CaN path is important to c-fos and c-myc expression. From above results the conclusion can be obtained that calcium signal transduction play an important role in the development of AS.