中文摘要：

目的：检测牙龈蛋白酶诱导成骨细胞凋亡中Bad的表达改变,为保护成骨细胞提供依据.方法：8.348U/L牙龈蛋白酶处理成骨细胞0、4、8、16、24、48和72h,或将不同浓度牙龈蛋白酶与成骨细胞作用24h后,蛋白质印迹法检测Bad蛋白表达改变.结果：在一定范围内,牙龈蛋白酶在诱导成骨细胞凋亡中呈时间和剂量依赖性上调Bad的表达,牙龈蛋白酶抑制剂TLCK有效抑制牙龈蛋白酶所诱导的Bad的高表达.结论：牙龈蛋白酶上调Bad的表达,促凋亡蛋白Bad参与了牙龈蛋白酶诱导成骨细胞凋亡过程.

英文摘要：

Objective： To examine the expression of Bcl-xl/Bcl-2-Associated Death promoter（Bad） during osteoblast apop- tosis induced by gingipains. Methods： Mouse osteoblast cell line MC3T3-E1 cells were cultured in the presence of 8.348U/L gingipains for 0, 4, 8, 16, 24, 48 and 72h, or in different doses ofgingipains（0, 4.174, 8.348 and 16.696 U/L） for 24h. Protein expression of Bad was determined by Western blotting. Results： In this study, gingipains upregulated the expression of Bad in a time- and dose- dependent manner in osteoblast apoptosis. Proteinase inhibitor TLCK effectively inhibited increased of Bad expression induced by gingipains. Conclusions： Bad would play a role in the process of os- teoblast apoptosis induced by gingipains.