中文摘要：

目的：通过酶消化法及流式细胞分选技术分选CD146＋牙周膜干细胞（Periodontal ligament cells，PDLCs），探讨其干细胞特性。方法：采用流式细胞技术从人牙周膜细胞中分选CD146＋PDLCs，并对其进行免疫组化染色（角蛋白、波形丝蛋白及Stro-1），成骨、成脂诱导分化及克隆形成实验鉴定其干细胞特性。结果：CD146＋PDLCs表达波形丝蛋白及Stro-1，具有成骨及成脂分化能力，体外培养能形成细胞克隆。结论：以CD146为分选指标的流式细胞分选技术可作为牙周膜干细胞筛选的方法。

英文摘要：

Objectives： To isolate and culture CD146 positive periodontal ligament cells （CD146＋PDLCs） by using type I collagenase digestion and flow cytometric sorting, in order to verify the stem cell properties of CD146＋PDLCs. Methods： CD146＋PDLCs were isolated by flow activated cell sorter fi＇om human PDLCs. The stem cell properties of CD146＋PDLCs were tested by immunohistochemical staining, osteogenic/adipogenic induction and colony-forming assay. Results： CD146＋PDLCs, expressing mesenchymal stem cell surface marker Stro-1, were able to form calcium nodules/lipid droplets under osteogenic/adipogenic induction and were capable of forming colonies. Conclusions： CD 146＋PDLCs shared the similar properties with periodontal ligament stem cells, which providing a new method for periodontal ligament stem cell isolation.