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毒死蜱降解菌枝孢霉菌的紫外诱变和筛选
  • 期刊名称:华南农业大学学报
  • 时间:0
  • 页码:44-48
  • 语言:中文
  • 分类:S482.7[农业科学—农药学;农业科学—植物保护] Q93[生物学—微生物学]
  • 作者机构:[1]华南农业大学昆虫毒理研究室,广东广州510642
  • 相关基金:国家自然科学基金(30871660); 广东省科技计划项目(2009B020310005)
  • 相关项目:果蔬拟除虫菊酯农药残留高效降解酶Cpde分子改造研究
中文摘要:

通过紫外诱变毒死蜱降解菌枝孢霉菌Cladosporium cladosporioidesHu-01,利用高效液相色谱(HPLC)检测,筛选获得12株突变菌株,其中突变株Hu-01-4和Hu-01-6的降解效果明显高于初始菌株Hu-01.突变株Hu-01-4和Hu-01-6经过加药斜面传种10代,Hu-01-6的降解率比初始菌株提高11.74%,并且保持良好的稳定性.突变菌株Hu-01-6在28℃、pH6.5培养条件下的生长量最大,降解率最高,且均明显高于初始菌株Hu-01.

英文摘要:

Twelve mutants were obtained from chlorpyrifos-degrading strain Cladosporium cladosporioides Hu-01 by UV-induced screening and their degradation capacities were determined by high performance liquid chromatography(HPLC).The degradation abilities of mutant strain Hu-01-4 and Hu-01-6 were higher than the original strain Hu-01.Furthermore,the mutant strain Hu-01-6 kept a good stability of degradation rate after 10 serial passages on the test-tube medium containing chlorpyrifos,increasing by 11.74% compared with the original strain.Moreover,both the growth and degradation ability of mutant strain Hu-01-6 were higher than original strain Hu-01 under the conditions of 28 ℃and pH 6.5.

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