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高效氯氰菊酯降解菌株HG-P-01的培养基筛选及优化
  • 期刊名称:微生物学通报
  • 时间:0
  • 页码:672-677
  • 语言:中文
  • 分类:S826.5[农业科学—畜牧学;农业科学—畜牧兽医] TQ453.292[化学工程—农药化工]
  • 作者机构:[1]华南农业大学昆虫毒理研究室农药与化学生物学教育部重点实验室,广东广州510642
  • 相关基金:基金项目:国家自然科学基金项目(No.30871660);广东省科技计划项目(No.20088020900007)
  • 相关项目:果蔬拟除虫菊酯农药残留高效降解酶Cpde分子改造研究
中文摘要:

通过比较查彼氏培养基、马铃薯葡萄糖培养基、理查德培养基、燕麦片培养基和玉米粉液体培养基等5种培养基对高效氯氰菊酯降解菌株Fusarium sp.HG.P-01生长的影响,确定了查彼氏培养基是菌丝生长最适宜的培养基。采用中心组分旋转设计技术,优化了查彼氏培养基中C、N、P的组成,以降解率为衡量指标,最佳配方包括C为20.94g/L、N为1.82g/L、P为1.66g/L,处理后24h对50mg/L高效氯氰菊酯期望降解率96.34%,HPLC法测定实际降解率为93.78%,对高效氯氰菊酯的降解率模型预测值与实测值一致。

英文摘要:

By means of comparing biomasses of biodegradation fungi, Fusarium sp. HG-P-01 for J3-cypermethrin, a synthetic pyrethroid insecticide used widely in China, in five different media, the Czapek-Dox medium was selected as the best medium for mycelia growth. Furthermore, an experiment of central composite rotatable design (CCRD) was used to optimize the content of nutrient components. The optimal composition of C, N and P in media for HG-P-01 were 20.94 g/L, 1.82 g/L and 1.66 g/L, respectively, in which an expectant or real rate of β-cyperrnethrin-degradation got to 96.34% or 93.78% by HPLC for a concentration of 50 mg/L after 24 h treatment. The predicted value in degradation rate model was consistent with that from HPLC method.

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