中文摘要：

目的观察Klotho基因（抗衰老基因）修饰的骨髓间充质干细胞（BMSC）移植对慢性心力衰竭大鼠心肌纤维化的影响。方法分离、培养、扩增大鼠骨髓间充质干细胞。慢病毒介导Klotho转染至BMSC中,逆转录-聚合酶链反应（RT-PCR）检测BMSC中Klotho mRNA的表达。制备大鼠慢性心力衰竭模型,随机分为正常对照组、模型对照组、增强型绿色荧光蛋白-BMSC组（EGFP-BMSC组）、EGFP-Klotho-BMSC组。细胞移植28天后多普勒超声检测心功能,荧光显微镜观察细胞存活及分布,HE染色观察心肌组织病理变化。Masson染色检测心肌胶原沉积含量和心肌纤维化程度。结果 Klotho转染BMSC后,BMSC中Klotho mRNA表达明显增多（P〈0.05）。移植28天后超声心动图结果显示,与EGFP-BMSC组及模型对照组比较,EGFP-Klotho-BMSC组左心室射血分数（LVEF）提高（P〈0.05）。EGFP-BMSC组及EGFP-Klotho-BMSC组心肌纤维化程度较模型对照组减轻（P〈0.05）,EGFP-KlothoBMSC组较EGFP-BMSC组心肌纤维化改善更为明显（P〈0.05）。结论 Klotho基因联合BMSC治疗可进一步抑制心肌纤维化,减少心肌间质胶原沉积,改善心功能。

英文摘要：

Aim To observe the effect of Klotho gene modified bone marrow mesenchymal stem cells （BMSC） transplantation on myocardial fibrosis in rats with heart failure.Methods Isolation,culture and amplification of rat bone marrow mesenchymal stem cells.Klotho transfection to BMSC and RT-PCR detection of Klotho mRNA expression in BMSC.The rat model of chronic heart failure were randomly divided into normal group,saline group,EGFP-BMSC group and EGFP-Klotho-BMSC group,with 6 rats in each group.The cardiac function was detected by Doppler ultrasound after 28 days of cell transplantation.The survival and distribution of the cells were observed by fluorescence microscope.The pathological changes of myocardial tissue were observed by HE staining.Masson staining was used to detect the content of myocardial collagen deposition and the degree of myocardial fibrosis.Results Klotho combined with BMSC treatment can better inhibit myocardial fibrosis,reduce myocardial interstitial collagen deposition and improve cardiac function.Conclusion Klotho gene combined with bone marrow mesenchymal stem cells could attenuate cardiomyocytes hypertrophy and restrain collagens hyperplasy in the progression of myocardial fibrosis.