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中文摘要：
目的：研究中波紫外线（UVB）诱导人皮肤成纤维细胞（FB）表达基质金属蛋白酶（MMP）-1和表没食子儿茶素没食子酸酯（EGCG）及c-Jun氨基端激酶（JNK）抑制剂SP600125对该诱导的保护作用。方法：以30mJ／cm。UVB及12．5μg／mLEGCG处理FB．同时以SP600125为对照，照光和（或）加药后于相应时间点提取上清及总RNA，以ELISA法检测MMP-1表达，反转录（RT）-PCR法检测细胞中MMP-1mRNA含量。结果：30mJ／cm。UVB照射FB后24hMMP-1表达为对照组的3．1倍，12h及24h时MMP-1mRNA表达分别增加至对照组的2．60倍及2．66倍（P均〈0．05）。UVB照射前、后加EGCG及SP600125组较单纯UVB照射组显著抑制MMP-1的表达．MMP-1mRNA含量分别为单纯照射组的71．9％及40．4％，MMP-1蛋白表达量分别为单纯照射组的61．8％及48．9％。结论：UVB照射FB后MMP-1mRNA及MMP-1表达水平均显著增加，EGCG可抑制UVB所致的MMP-1mRNA及MMP-1高表达。
英文摘要：
Objective： To explore the expression of MMP-1 after UVB irradiation on fibroblasts and the photoprotection ef leers of EGCG and JNK inhibitor. Methods： The fibroblasts were treated with 12.5 μg/mL EGCG and irradiated by 30 mJ/cm2 UVB. The conditioned supernatants and RNA of fibroblasts were extracted at propotional time points after treated with EGCG or JNK inhibtor. ELISA and RT-PCR were employed to detect the expressions of MMP-1 and MMP-1 mRNA respectively. Results： The expressions of MMP-1 protein of 24 h were increased 3.1 times, while MMP-1 mRNA of 12 h and 24 h after 30 mJ/cm2 UVB irradiation were increased 2.60 and 2.66 times respectively （P〈0.05）. MMP-1 mRNA and MMP-1 protein were decreased to 71.9%, 40.4% and 61.8%, 48.9% of the fibroblasts treated with EGCG and JNK inhibitor. Conclusion： MMP-1 mRNA was increased significantly after UVB irradiation. EGCG could decrease the upregnlation of MMP-1 mRNA and MMP-1 protein induced by UVB irradiation.
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