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中文摘要：
目的：研究中波紫外线（UVB）慢性辐射BALB／C小鼠后皮肤的组织病理改变、表皮细胞凋亡情况及表没食子儿茶素没食子酸酯（EGCG）对其的影响。方法：EGCG局部外用于小鼠耳、背部皮肤后分别给予不同强度的UVB辐射，每日1次．连续1个月，石蜡切片苏木精-伊红染色观察不同处理条件下皮肤组织病理变化，同时应用末端转移酶介导的缺口末端标记（TUNEL）法检测小鼠表皮中的凋亡细胞。结果：UVB慢性辐射对BALB／C小鼠皮肤有明显影响，主要表现有表皮过度角化、棘层肥厚、海绵样水肿、晒斑细胞、真皮乳头层水肿、毛细血管扩张、炎性细胞浸润等。EGCG预处理能减轻UVB诱导的上述组织病理变化。另外，对慢性低剂量UVB辐射组，EGCG有一定的促细胞凋亡作用。结论：不同剂量UVB慢性辐射后小鼠皮肤组织病理改变明显，EGCG可保护UVB辐射诱导的光损伤作用，并对低剂量慢性UVB辐射后BALB／C小鼠皮肤细胞有促进凋亡作用。
英文摘要：
Objective： To investigate the pathological changes and the apoptosis of epidermal cells on the skin of BALB/C mouse induced by chronic ultraviolet B （UVB） irradiation and the photoprotective effect of topical application with （-）-epigallocatechin-3-gallate （EGCG） against it. Methods： The skin of the ears and the back of BLAB/C mice was pretreated with EGCG and then irradiated with different doses of UVB daily for one month. The paraffin sections of skin specimens were stained with hematoxylin-eosin stain for observing the pathological changes. The apoptotie cells were detected by TUNEL. Results： There were obvious pathological changes in the skin of BALB/C mouse after chronic UVB irradiation, they were hyperkeratosis, acanthosis, spongiosis, sunburn cells in the epidermis, edema in the papillary dermis, telangiectasia and inflammatory cell infiltration in the dennis. These UVB-induced histopathological changes could be reduced by the pre-intervention with EGCG. In the group of low-dose-UVB irradiation, enhanced apoptosis by EGCG was observed. Conclusions： There are marked histopathological changes in the skin of BALB/C mice after the chronic UVB irradiation with different doses of UVB. Topical application of EGCG on the skin of BALB/C mice can prevent UVB-induced photodamage, and promote the epidermal cell apoptosis induced by the low dose irradiation of UVB.
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