中文摘要：

目的观察电针对易卒中型肾性高血压大鼠（RHRSP）大脑中动脉闭塞（MCAO）后不同时间点大脑皮层、延髓、颈髓区中枢神经髓鞘相关生长抑制因子Nogo—A mRNA表达的影响，探讨其对急性脑梗死远隔损害的保护机制。方法应用随机化原则，对SD雄性大鼠采用双肾双夹术复制成RHRSP模型及采取线栓法制作成MCAO模型，共分为高血压组、假手术组、模型组、电针组、假针刺组（每组各60只），其中高血压组为单纯RHRSP模型，假手术组仅做手术创伤不行MCAO，模型组、电针组、假针刺组分别在RHRSP模型和MCAO模型基础上不处理、选取督脉“百会”和“大椎”穴进行电针治疗（1次／d，共28d）以及将针灸针贴于上述相同穴位处皮肤。治疗第1、7、14、28天分别分离各组相应数量大鼠右侧大脑皮层、延髓和左侧颈髓，采用RT-PCR法检测Nogo-A mRNA的表达。结果（1）皮层区：与高血压组比较，治疗第7、14、28天模型组、电针组、假针刺组Nogo-A mRNA表达明显升高，差异有统计学意义（P〈0．05）；与模型组比较，治疗第7、14、28天电针组Nogo—A mRNA表达明显降低，差异有统计学意义（P〈0．05）。（2）延髓区：与高血压组比较，治疗第14、28天模型组、电针组、假针刺组Nogo—A mRNA表达明显升高，差异有统计学意义（P〈0．05）；与模型组比较，治疗第14、28天电针组Nogo—A mRNA表达明显降低，差异有统计学意义（P〈0．05）。（3）颈髓区：与高血压组比较，治疗第28天模型组、电针组、假针刺组Nogo—A mRNA表达明显升高，差异有统计学意义（P〈0．05）；与模型组比较，治疗第28天电针组Nogo-A mRNA表达明显降低，差异有统计学意义（P〈0．05）。结论大脑皮层、延髓、颈髓区Nogo-A mRNA表达增高是急性脑梗死远隔损害发生的一个重要原因．电针对高血压性脑梗死大鼠的保护作用可能

英文摘要：

Objective To observe the effect of electric acupuncture on Nogo-A mRNA expression in the cortex, medulla oblongata and cervical cord areas of models of stroke-prone renovascular hypertensive rats （RHRSP） after middle cerebral artery occlusion （MCAO） at different time points, and investigate the possible mechanisms of electric acupuncture in diaphragma damage in ischemic stroke. Methods Male SD rats were divided into hypertension group, sham-operated group, model group, electric acupuncture group and sham electric acupuncture group by random number table method （n=60）. Rats of hypertension group were the pure RHRSP models; rats of sham-operated group only received surgical trauma; rats of model group received no treatment based RHRSP and MCAO; ＂Baihui＂ and ＂Dazhui＂ acupoints were electroacupuncted in rats of electric acupuncture group for 28 days, once a day; in sham electric acupuncture group, acupuncture needles were sticked on the skin of rats where ＂Baihui＂ and ＂Dazhui＂ locate. One, 7, 14, 28 days after treatment, the right side of the cortex,medulla oblongata and the left side of the cervical cord were separated in a certain number of each group rats. RT-PCR was employed to detect the Nogo-A mRNA expression. Results （1） Cortex area： on the 7^th, 14^th, 28^th days, model group, electric acupuncture group and sham electric acupuncture group had higher Nogo-A mRNA expression than hypertension group with statistically significant difference （P〈 0.05）; as compared with the model group, electric acupuncture group had statistically lower Nogo-A mRNA expression on the 7^th, 14^th, 28^th days （P〈0.05）. （2） Medulla oblongata area： on the 14^th and 28^th days, model group, electric acupuncture group and sham electric acupuncture group had higher Nogo-A mRNA expression than hypertension group with statistically significant difference （P〈0.05）; as compared with the model group, electric acupuncture group had statistically lower Nogo-A mRNA expression on the 14^th a