中文摘要：

目的：观察电针对易卒中型肾性高血压大鼠（RHRSP）大脑中动脉闭塞（MCAO）后脑缺血/再灌注（I/R）不同时间点脑梗死灶体积与皮质、脊髓神经元数目的影响，探讨电针对脑梗死远隔损害的可能机制。方法将480只雄性SD大鼠行双肾双夹术复制RHRSP模型，采取尾动脉测压术筛选制模成功大鼠370只，按随机数字表法分为高血压组60只、假手术组60只，再将剩余制模成功大鼠用线栓法复制MCAO模型，采用Longa 5分制法，在动物麻醉清醒后进行神经功能缺损程度评分（NDS），分值在1～3分的大鼠纳入实验。共有190只大鼠MCAO制模成功，从中随机抽取10只进行2，3，5-氯化三苯基四氮唑（TTC）染色确定梗死范围，将剩余的180只MCAO大鼠按随机数字表法分为模型组、电针组、假针刺组，每组60只。假手术组仅进行手术创伤；电针组在制模当天选取督脉百会和大椎穴进行电针治疗，每日1次，共28 d；假针刺组选大椎、百会穴位处给予针灸针贴肤治疗。治疗后1、7、14、28 d分别处死各组大鼠，取大鼠脑组织进行尼氏染色，检测梗死灶体积与神经元数目。结果①脑组织梗死灶体积：高血压组、假手术组未发现有梗死灶。MCAO术后1 d、7 d，模型组、电针组和假针刺组大鼠脑梗死灶体积均逐渐增大〔1 d 3组分别为（12.36＆#177;0.11）%、（12.19＆#177;0.15）%、（12.24＆#177;0.16）%，7 d 3组分别为（20.01＆#177;0.24）%、（19.54＆#177;0.61）%、（19.77＆#177;0.25）%〕，14 d、28 d，模型组、电针组和假针刺组大鼠脑梗死灶体积均有缩小趋势〔14 d 3组分别为（17.18＆#177;0.23）%、（16.96＆#177;0.11）%、（17.08＆#177;0.62）%，28 d 3组分别为（14.38＆#177;0.21）%、（13.95＆#177;0.46）%、（14.29＆#177;0.37）%〕，但3组比较差异均无统计学意义（均P＞0.05）。②皮质区：MCAO术后1 d各组间皮质神经元数目比较差异均

英文摘要：

Objective To observe the effect of electric acupuncture on the infarct volume and amount of cerebral cortex and spinal neuron at different times of cerebral ischemia/reperfusion（I/R）in stroke prone renovascular hypertensive rats（RHRSP）with middle cerebral artery occlusion（MCAO）,and investigate the possible mechanisms of electric acupuncture on remote damage in ischemic stroke. Methods 480 male SPF Sprague-Dawley（SD）rats were duplicated to form the RHRSP models by clamping both kidneys. 370 successful ones were selected by taking the tail artery blood pressure,and divided into hypertension group and sham operation group（each n=60）by random number table method. The MCAO models were created by stringing middle cerebral artery in the remaining RHRSP. The nerve function defect score（NDS）was graded by Longa 5 point method after the rats waked up from anesthesia,then the ones scored 1-3 were enrolled. Totally,there were 190 rats with MCAO successfully created from which 10 were randomly selected to determine the infarct size by 2,3,5-triphenyl four azole nitrogen chloride（TTC）staining. The remaining 180 MCAO rats were randomly divided into model group,electric acupuncture group and fake acupuncture group（each n=60）. The sham operated group only received surgical trauma;the electrical acupunctures at＆quot;Baihui＆quot;and＆quot;Dazhui＆quot;acupoints on Du channel were performed on the day of model establishment in electric acupuncture group,once a day for 28 days;in fake acupuncture group,sticked the acupuncture needles at the skin of＆quot;Baihui＆quot;and＆quot;Dazhui＆quot;points,then gived the same electrical acupuncture treatment. On 1,7,14 and 28 days after treatment,the rats of each group were respectively sacrificed,and the brains were collected,then the infarct volume and spinal neuron number were calculated by Nissl staining. Results ①Cerebral infarction volume：No infarcts were found in hypertension group and sham operated group. On 1 day and 7 days after MCAO,the i