RNA干扰(RNAinterference,RNAi)是通过双链RNA介导,特异性地降解相应序列的mRNA,从而导致转录后水平的基因沉默。近年来,利用小分子RNA(small interfering RNA,si RNA)介导的RNAi技术已越来越多的应用于哺乳动物细胞中。同时,在活体中利用RNAi研究多种生物基因功能也成为一种有效的手段。文章旨在介绍在转基因小鼠中RNAi的应用。
RNA interference degrades mRNA specifically and potently though the mediation of corresponding double-stranded RNA and leads to post-transcriptional gene silencing.Recently,small interfering RNA duplexes(siRNA) is used in mammalian cells to silence gene expression.At the same time,RNAi is a powerful tool for studying gene function in vivo.This review provides an overview of transgenic RNA interference in mice.