中文摘要：

目的分析大鼠LHβ mRNA表达的促性腺激素释放激素（GnRH）受体后信号转导机制。方法将体外培养的大鼠腺垂体促性腺激素（GTH）细胞用cAMP的兴奋剂FSK或抑制剂SQ22536处理后,再用高频GnRH脉冲刺激,然后用实时荧光定量PCR法测定细胞LHβ mRNA的Ct值,并与空白组比较。结果 LHβ mRNA的Ct值随着GTH细胞cAMP含量的增高而显著降低,随着cAMP含量的降低而显著增高。结论 cAMP是高频GnRH脉冲刺激所引起的LHβ mRNA表达的受体后的信号转导途径。

英文摘要：

Objective Try to analyze the gonadotropin-releasing hormone （GnRH） post-receptor signal transduction mechanism of the expression of LHI3 mRNA in Rats. Methods The gonadotropic hormone （GTH） cells of rat adenohypophysis was stimulated by GnRH impulse at high frequency after cAMP in them was excited by forskolin （FSK） or inhibited by SQ 22536. The Ct value of LHβ mRNA was detected by real-time quantitative polymerase chain reaction, and was compared with that of the blank control group. Results The Ct value of LHβ mRNA was significantly falling with the rise of cAMP in GTH ceils and significantly rising with the falling of cAMP. Conclusions The cells stimulated by GnRH impulse at high frequency respond to LH βmRNA and cAMP is the post-receptor signal transduction pathway.