中文摘要：

通过N-甲基-N′-硝基亚硝基胍、8-氮鸟嘌呤、6-巯基鸟嘌呤紫外线照射的联合作用,试图建立C6细胞的次黄嘌呤鸟嘌呤磷酸核糖基转移酶缺陷株,为建立大鼠垂体促性腺激素杂交瘤细胞株奠定基础,但最终未能获得预期的能够被HAT培养基筛选的HGPRT-缺陷株,提示C6细胞株的HGPRT基因非常稳定,诱变方法难以使其突变缺失。

英文摘要：

This study tries to product rat gloma cells hypox anthine-guaninep hosphoribosyl transferase deficiency lines by combined effects of N-methyl-N′-nitro-nitrosoguanidine,8-azaguanine,6-thioguanine and ultraviolet irradiation in order to establish rat pituitary gonadotropic hormone hybridoma cell lines in future,but wo are not able to gain the expectant glioma HGPRT-cell lines which can be sieved by hypoxan thine-aminop terinthymidine selective medium.The experiment indicate that the HGPRT gene of C6 cells is too stabile to be induced deficient on this experiment conditions.