利用制备好的PCV2Cap蛋白,将其与弗氏佐剂乳化,免疫Balb/c鼠,制备多克隆抗体并对其抗体效价进行鉴定和分析抗体产生规律。结果表明,制备的多克隆抗体效价达到1:12800,该规律的发现为单克隆抗体制备免疫程序的制定奠定了试验基础。
The expressed PCV2 Cap protein mixed with adjuvants immuned Balb/c rats, in order to produce polyclonal antibodies, identified its antibody titers and analysed antibodies produced rules. The results showed that the titers of polyclonal antibody reach to 1:12 800. This rule settle experiment basis of monoclonal antibody preparation immunized produce.