中文摘要：

目的观察重组质粒pIRES-fimA：IL15经滴鼻免疫BABL／c小鼠后诱导产生的血清和唾液抗体反应及白细胞介素-15（IL-15）对sigA反应的调节作用。方法重组质粒pIRES-fimA：IL15与pIRES-fimA经滴鼻免疫和肌肉注射免疫BABL／cdx鼠，以间接ELISA方法检测血清中IgG和唾液中sIgA抗体水平。结果重组质粒pIRES-fimA：IL15与pIRES-fimA经滴鼻免疫可诱导产生唾液sigA反应，且该反应明显高于肌肉注射组，血清IgG水平与肌肉注射组无统计学差异。pIRES-fimA：IL15经滴鼻免疫产生的唾液sIgA滴度显著高于pIRES-fimA组，且有统计学差异（P〈0．05）。结论滴鼻免疫可以作为抗牙龈卟啉单胞菌DNA疫苗有效的黏膜免疫途径，诱导循环和口腔局部抗体反应：IL-15可以作为细胞因子佐剂通过滴鼻途径增强该疫苗诱导的sIgA反应强度。

英文摘要：

Objective To observe the antibody responses induced by recombinant plasmid pIRES-fimA：IL15 via nasal immunization to BABL/c mice and the regulation of IL-15 to sIgA. Methods BABUc mice were immunized with recombinant plasmids pIRES-fimA：IL15 and pIRES-fimA via nasal or intramuscular route. Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA. Results Nasal immunization with plasmids pIRES- fimA：IL15 or pIRES-fimA elicited significant higher level of salivary FimA-specific sIgA responses compared with intramuscular immunization. There was no significant difference of the serum IgG responses between nasal immunization mice and intramuscular immunization mice. Nasal immunization with plasmid pIRES-fimA：IL15 elicited significant higher level of salivary sIgA response than with pIRES-fimA （P〈0.05）. Conclusion Nasal dropping may be an effective mucosal immunization route of anti-Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region. IL-15 has a positive regulation effect to sIgA response.