中文摘要：

360只1日龄天府肉鸭随机分为6组，分别喂以对照日粮（Cu 8 mg/kg）和高铜日粮（Cu 100 mg/kg，高铜Ⅰ组；Cu 200 mg/kg，高铜Ⅱ组；Cu 400 mg/kg，高铜Ⅲ组；Cu 600 mg/kg，高铜Ⅳ组；Cu 800 mg/kg，高铜Ⅴ组）6周，以流式细胞术研究高铜对雏鸭肾脏细胞周期和凋亡的影响。结果显示，肾脏细胞的调亡比例随日粮铜水平的升高而升高，当日粮铜水平达到400 mg/kg以上时，肾脏细胞凋亡比例与对照组比较差异极显著（P〈0.01）。日粮铜水平达到400 mg/kg以上时，肾脏细胞的增殖指数（PI）极显著低于对照组（P〈0.01）。结果表明日粮铜水平达到400 mg/kg以上时会引起肾脏受损，肾脏细胞的增殖分化严重受阻，肾脏细胞凋亡大量增加。

英文摘要：

The experiment was conducted with the objective of examining the effect of dietary high copper on the cell cycle and apoptosis of kidney in ducklings by the methods of flow cytometry （FCM）. 360 one-day-old Tianfu meat ducklings were randomly divided into six groups and fed on diets as follows： Control （Cu 8 mg/kg） and high copper （Cu 100 mg/kg,high copper group Ⅰ;Cu 200 mg/kg, high copper group Ⅱ;Cu 400 mg/kg, high copper group Ⅲ;Cu 600 mg/kg, high copper group Ⅳ ;Cu 800 mg/kg,high copper group Ⅴ） for six weeks. Kidney samples were collected, then the cell cycle and apoptosis were tested by FCM. The results showed that the percentage of renal cell apoptosis was increased in high copper groups Ⅲ, Ⅳ and Ⅴ when compared with that of control group. The G0/G1 phase of cell cycle of renal cell was lower, and S, G2＋M phase was higher in high copper groups Ⅰ and Ⅱ than that in control group. The G0/G1 phase of cell cycle of renal cell was much higher, and S, G2＋M phase was lower in high copper groups Ⅲ , Ⅳ and Ⅴ than that in control group （ P〈0.05 or P〈0.01）. It is concluded that dietary copper in excess of 400 mg/kg diet impaired the progression of renal cells from G0/G1 phase to S phase obviously and induced renal cell apoptosis.