综述了近几年来在微流控芯片上以检测或动力学研究为目的而进行的各种生物化学反应技术。包括酶反应、免疫反应、酶联免疫反应、PCR反应和含氮氧化物释放反应。对非均相反应的蛋白质在微管道中的固定技术、均相反应的管道设计技术以及在微流控芯片上集成生化反应的优越性等做了详细的讨论。
Different kinds of biochemical reaction approaches for the goal of detection and kinetics studies on microfluidic chips are reviewed, including enzymatic assay, immunoassay, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and nitric oxide release reaction. According to the very characteristics of certain biochemical reaction, immobilization of proteins on channel wall for inhomogeneous reactions, channel network design for homogeneous reactions and the advantages of performing biochemical reactions on microfluidic chips are investigated in details.