中文摘要：

目的：研究苏芪浸膏在抗小鼠Lewis肺癌转移过程中对T淋巴细胞因子的影响，探讨苏芪浸膏在调节肿瘤炎性微环境中的作用。方法：将100只C57BL／6小鼠随机分为空白对照组、荷瘤模型组、环磷酰胺组、苏木组、苏芪浸膏组，除空白对照组外每只小鼠右腋皮下接种0．2mLLewis肺癌细胞悬液，观察各组小鼠Lewis肺癌肺转移抑制率；流式细胞术测各组小鼠脾CD4^4淋巴细胞中Th17/Treg细胞百分比的动态变化；ELISA法检测各组小鼠脾CD4^4淋巴细胞培养上清中白细胞介素-17（IL-17）、IL-23、干扰素-γ（IFN-γ）、IL_4、IL-2、IL-6动态水平；RT-PCR法检测各组小鼠CD4^4淋巴细胞Foxp3mRNA及RORγtmRNA的动态表达水平。结果：就肺转移抑制率而言，苏芪浸膏组优于苏木组（P〈0．05），而与环磷酰胺组相似；各荷瘤组小鼠脾Th17／Treg随时间迁移呈上升趋势，除环磷酰胺组外，其它荷瘤组与苏芪浸膏组比较，差异均有统计学意义（P〈0．05）；Th17细胞的增殖刺激因子IL-23及效应因子IL-17，以及Thl7细胞的关键转录因子RORγtmRNA表达水平呈现出与Thl7细胞相应的动态改变；Treg细胞的增殖刺激因子IL-6，以及Treg细胞的关键转录因子Foxp3mRNA表达水平呈现出与Treg细胞相应的动态改变。荷瘤模型组ILl4、IL-6较空白对照组有升高趋势，经苏芪浸膏和环磷酰胺治疗后，在第21天时与荷瘤模型组比较，差异有统计学意义（P〈0．05）；荷瘤模型组IL-2、IFN-γ与空白对照组比较有下降趋势，经苏芪浸膏治疗后，在第21天时与荷瘤模型组比较，差异有统计学意义（P〈0．05）。结论：苏芪浸膏抗Lewis肺癌转移的作用机制部分可能是通过调控炎性微环境、抑制肿瘤局部炎性反应、增强机体免疫功能而实现的。

英文摘要：

Objective： By study the effects of Suqi extract on T lymphocyte factors in metastasis of mice with lewis lung cancer, to explore the role of Suqi extract in regulating the tumor inflammatory microenvironment. Methods： 100 C57BL/6 mice were randomly divided into five groups： blank control group, tumor-bearing model group, cyclophosphamide group, sappan wood group and Suqi extract group. Except for the blank control group, the other groups were subcutaneously inoculated 0.2mL Lewis lung carcinoma cell suspension in the right axillary. At 14 and 21 days after modeling, the lung metastasis inhibitory rate of each group was observed by immunohistochemistry. The dynamic changes of the percentage of Thl7/Treg cells in the mice spleen CD4^ T lymphocytes were observed by flow cytometry. The dynamic levels of IL-17, IL-23, IFN- γ, IL-4, IL-2 and 1L-6 in the supematant of CD4^T lymphocytes of mice were detected by ELISIA. The dynamic expression levels of ROR γ/t and Foxp3 mRNA of CDa^T lymphocytes were detected by RT-PCR. Results： The lung metastasis inhibitory rate in Suqi extract group was superior to that in sappan wood group （P〈0.05）, which was similar to that in the cyclophosphamide group. Thl7/Treg cells in all tumor-bearing groups increased as it evolved over time. There was a statistical significance in Thl7/Treg cells between the tumor- bearing groups （except for cyclophosphamide group） and the Suqi extract group （P〈0.05）. IL-23 that the proliferation stimulating factor of Thl7 cell, IL-17 that effector molecule of Thl7 cell and ROR γ/t that key transcription factor of Thl7 cell, the mRNA expressions of those were related to the dynamical changes of Thl7 cells. IL-6 that the proliferation stimulating factor of Treg cells and Foxp3 that key transcription factor of Treg cells, the mRNA expressions of those were related to the dynamical changes of Treg cells. The levels of 1L-4 and IL-6 in tumor-bearing groups showed a rising trend compared with that in blank control group.But, after being t