中文摘要：

目的探究复方苦参注射液对人肝癌SMMC-7721细胞凋亡的影响。方法无菌培养人肝癌细胞SMMC-7721，采用MTT法检测复方苦参注射液对SMMC7721细胞的影响，复方苦参注射液的浓度分别为3．34、2．38、1．43和0．48mg／ml；采用膜联蛋白V-异硫氰酸荧光素（AnnexinV-FITC）／碘化丙啶（propidium iodide，PI）双染法测肝癌细胞凋亡率，复方苦参注射液的浓度分别为0．86、1．72和3．43mg／ml。结果复方苦参注射液浓度3．34、2．38、1．43、0．48mg／ml作用于SMMC．772124h的细胞抑制率分别为51．03％、53．67％、49．83％、0．03％；作用48h后，上述浓度的细胞抑制率分别为67．14％、65．35％、62．25％、0．05％，作用72h后，分别为74．16％、68．77％、66．04%、26．02％。结果复方苦参注射液浓度0．86、1.72和3．43mg／ml作用于SMMC．772124h的细胞凋亡率分别为（2．86±0．35）％、（15．16±1．15）％、（13．17±0．40）％；作用48h后，上述浓度的细胞凋亡率分别为（8．57±0．44）％、（28．07±0．76）％、（29．81±8．10）％，均高于空白对照组（1．05±0．09）％（P〈0．05）。结论复方苦参注射液对人肝癌SMMC．7721细胞增殖具有抑制作用，其作用机制与诱导细胞凋亡有关。

英文摘要：

Objective To explore the compound matrine injection on human hepatoma smmc-7721 cells＇ proliferation. Methods Human hepatoma smmc-7721 cells were sterilely cultured. Assayed the effects of compound matrine injection on smmc-7721 cells by MTT. The concentrations of the compound matrine injection were 3.34, 2.38, 1.43 and 0.48 mg/ml; Used the double staining method of annexin Vfluorescein isothiocyanate （Annexin V-FITC）/propidium iodide （propidium iodide, PI） to measure the apoptosis of hepatoma cell. The concentrations of the compound matrine injection were 0.86, 1.72, and 3.43 mg/ml. Results The apoptosis rates of compound maUine injection with different concentration of 3.34, 2.38, 1.43 and 0.48 mg/ml on liver cancer cell SMMC-7721 wereS1.03%, 53.67%, 49.83% and 0.03% respectively after 24 hours; and 67.14%, 65.35%, 62.25% and 0.05% respectively after 48 hours;and 74.16%, 68.77%, 66.04% and 26.02% respectively after 72 hours. The apoptosis rates of compound matrine injection with different concentration of 0.86, 1.72 and 3.43 mg/ml on liver cancer cell SMMC-7721 were（2.86±0.35）%, （15.16±1.15）% and（13.174±0.40）% respectively after 24 hours; and （8.574±0.44）%, （28.074±0.76）% and （29.814-8.10）% respectively after 48 hours, which were significantly higher than the control group （1.05 4± 0.09）% （P±（0.05）. Conclusion The compound matrine injection has a significant inhibition on human hepatoma smmc-7721 cells＇ proliferation and the mechanism was its inducing cell apoptosis.