中文摘要：

背景：天然甲壳素溶解性差，不易加工处理。传统工艺制备的甲壳素存在较多致炎性杂质蛋白。 目的：观察由高纯度壳聚糖乙酰化制备的甲壳素材料与许旺细胞的体外生物相容性。 设计、时间及地点：对比观察实验，于2006—09／2007—10在南通大学医学院组织学与胚胎学教研室及江苏省神经再生重点实验室完成。 材料：新生一两天的SPF级SD大鼠坐骨神经和臂丛神经用于培养许旺细胞，自制壳聚糖膜，脱乙酰度为92．5％。 方法：将脱乙酰度92．5％的壳聚糖膜浸入5％的醋酐甲醇溶液中进行乙酰化反应后制备甲壳素膜。体外接种许旺细胞分别至壳聚糖膜和甲壳素膜上。 主要观察指标：共培养6d后通过抗S-100蛋白免疫细胞化学的方法鉴定，并进行光镜、扫描电镜观察细胞形态。CCK-8试剂检测在壳聚糖膜浸出液、甲壳素膜浸出液、L-15培养基、有机锡浸出液中生长2，4，6d许旺细胞的细胞活力。 结果：抗S-100蛋白免疫细胞化学的鉴定结果提示这类细胞为许旺细胞；光镜、扫描电镜观察均发现细胞能够在壳聚糖膜和甲壳素膜表面正常生长，细胞贴附牢固，外形饱满，大多数细胞呈长梭形，边缘清晰，细胞增殖情况良好。细胞活力检测的结果显示壳聚糖膜浸出液和甲壳素膜浸出液中生长的许旺细胞与L-15培养基中生长的细胞相比，细胞活力无明显差异（P〉0．05）。有机锡浸出液中生长的许旺细胞活力均低于其他浸出液，差异有显著性意义（P〈0．05）。 结论：壳聚糖乙酰化制备的甲壳素材料与许旺细胞体外生物相容性良好。

英文摘要：

BACKGROUND： Natural chitin is poor dissoluble, thus hinders the further processing. Chitin prepared by traditional technology holds many impure proteins that induce inflammation. OBJECTIVE： To evaluate the biocompatibility between Schwann cells and chitin that acetylated from high purity chitosan in vitro. DESIGN, TIME AND SETTING： This experiment was designed as comparative observation, and carried out in Department of Histology and Embryology, Medical College of Nantong University and Key Laboratory of Neuroregeneration of Jiangsu Province from September 2006 to October 2007. MATERIALS： Neonatal SD rats, 1-2 days old and SPF grade, were used as donors, the sciatic nerve and brachial plexus nerve were harvested to culture Schwann cells; Chitosan membrane was self-made into a deacetylation degree of 92.5%. METHODS： Chitosan membrane （degree of deacetylation： 92.5%） was immersed in 5% acetic anhydride of methanol solution. The Schwann cells were co-cultured with chitosan membrane and chitin membrane, respectively. MAIN OUTCOME MEASURES： Subsequent to the co-culture, Schwann cells were identified by immunocytochemical S-100 staining, the morphology of Schwann cells was observed by light and scanning electron microscopy. Cell viabilities of Schwann cells cultured in the extractions of chitosan membrane, chitin membrane, L-15 medium and organotin for 2, 4, 6 days were evaluated by CCK-8. RESULTS： The results of immunocytochemistry S-100 staining suggested that most of these cells were Schwann cells. Schwann cells could grow onto chitosan membrane and chitin membrane normally, most of these cells were long olivary, both the cell multiplication and adhesion was good. The measurement of cell viabilities indicated that there was no significant difference in the Schwann cells cultured in the extraction of chitosan membrane and chitin membrane compared with L-15 medium （P 〉 0.05）. the cell viabilities of the Schwann cells cultured in organotin extraction were significantly decreased ?